Phylogenetic trees of mitochondrial DNA sequence analyses for the Caligidae. A) Maximum likelihood tree constructed from analysis of 462 characters of aligned small subunit 16S ribosomal gene. B) Bayesian topology based on analysis of 528 characters of aligned CO1 genes of 25 caligid taxa. Pseudocaligus brevipedes is robustly supported in a clade with C. curtus and C. centrodonti in both analyses (node a, shaded boxes). Pseudocaligus fugu forms the basal taxon to the C. elongatus group in the 16S analysis and is unresolved in the CO1 tree. Pseudocaligus uniartus is unresolved in both analyses. Lepeophtheirus spp. form well-supported clades in both trees (node b, green boxes), apart from L. natalensis (highlighted) in the CO1 tree that is unresolved. Thick branches are from nodes with full support and figures at the nodes represent bootstrap support values from 1000 samplings for the 16S tree and posterior probabilities for the Bayesian topology.