Analysis of surface proteins from T. spiralis muscle larvae. (A) SDS-PAGE analysis of surface proteins. Protein samples were separated in 12% polyacrylamide gels under reducing conditions, and the gels were stained with Colloidal Coomassie blue G-250. M, low molecular weight protein marker; Lane 1, surface proteins; Lane 2, ES proteins; Lane 3, somatic proteins from whole larvae. (B) Western blotting of surface proteins. The surface proteins were recognize were recognized by sera from mice infected with T. spiralis at 18 dpi (Lane 1) and at 42 dpi (Lane 2) and sera from mice immunized with surface proteins (Lane 3), not by sera from normal mice (Lane 4).