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Table 1 Identification of T. spiralis muscle larval surface protein by MALDI-TOF/TOF-MS

From: Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry

Spot no. Protein name Accession no. Theoretical Mr/pIa MOWSE score Coverage (%) No. matched peptides p-value
1 partial P49 antigen gi|162542 34.5/5.23 80 15 3 0.00032
2 Not identified - - - - - -
3 hypothetical protein Tsp_08444 gi|339247637 18.9/9.70 62 57 7 0.023
4 deoxyribonuclease II family protein gi|339241449 38.1/5.95 98 10 2 6.8e-006
5 serine protease gi|168805931 35.7/5.97 245 18 4 1.1e-020
6 deoxyribonuclease II family protein gi|339241449 38.1/5.95 382 22 6 2.1e-034
7 deoxyribonuclease II family protein gi|339241449 38.1/5.95 315 22 6 1.1e-027
8 Not identified - - - - - -
9 deoxyribonuclease II family protein gi|339241449 38.1/5.95 447 28 7 6.6e-041
10 ps73f07.y1 T. spiralis adult pAMP1 v1 T. spiralis cDNA 5′, mRNA sequence gi|21410467 22.3/4.83 371 39 9 2e-032
11 ps97f02.y1 T. spiralis adult pAMP1 v1 T. spiralis cDNA 5′, mRNA sequence gi|21414022 20.7/4.71 369 38 9 3.2e-032
12 PREDICTED: keratin, type II cytoskeletal 1 [Gorilla gorilla gorilla] gi|160961491 66.0/8.15 232 36 12 1.4e-016
13 serine protease gi|168805933 48.7/6.33 145 9 2 1.1e-010
14 serine proteinase gi|13641204 48.7/6.33 116 12 5 8.4e-008
15 deoxyribonuclease II family protein gi|339241449 38.1/5.95 312 30 9 2.1e-027
16 53 kDa excretory/secretory antigen gi|805126 4.71/8.42 280 21 8 3.3e-024
17 conserved hypothetical protein gi|339258426 13.5/4.54 121 19 2 2.6e-008
18 Not identified - - - - - -
19 conserved hypothetical protein gi|339258426 13.7/4.54 209 29 3 4.2e-017
20 conserved hypothetical protein gi|339258426 13.5/4.54/ 205 42 4 1.1e-016
21 conserved hypothetical protein gi|316966355 13.5/4.54 92 30 2 2.2e-005
22 conserved hypothetical protein gi|316966355 13.5/4.54 70 19 2 0.0035
23 conserved hypothetical protein gi|339258426 13.5/4.54 231 29 3 2.6e-019
24 conserved hypothetical protein gi|339258426 13.5/4.54 254 59 5 1.3e-021
25 conserved hypothetical protein gi|339258426 13.54.54 248 59 5 5.3e-021
26 Not identified - - - - - -
27 MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence gi|13198997 19.9/4.76 345 38 6 8.1e-030
28 MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence gi|13198997 19.9/4.76 359 38 6 3.2e-031
29 Not identified - - - - - -
30 Not identified - - - - - -
31 MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence gi|13198997 19.9/4.76 423 55 6 1.3e-037
32 TPAF-aac52c09.g1 T. spiralis _EST T. spiralis cDNA 5′, mRNA sequence gi|157957485 21.6/9.48 382 57 7 1.6e-033
33 ps19d01.y1 T. spiralis ML CMVsport jasmer T. spiralis cDNA 5′, mRNA sequence gi|148310851 17.7/10.32 223 53 6 1.3e-017
  1. aTheoretical molecular mass (kDa) and isoelectric point (pI).