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Table 1 Identification of T. spiralis muscle larval surface protein by MALDI-TOF/TOF-MS

From: Proteomic analysis of surface proteins of Trichinella spiralis muscle larvae by two-dimensional gel electrophoresis and mass spectrometry

Spot no.

Protein name

Accession no.

Theoretical Mr/pIa

MOWSE score

Coverage (%)

No. matched peptides

p-value

1

partial P49 antigen

gi|162542

34.5/5.23

80

15

3

0.00032

2

Not identified

-

-

-

-

-

-

3

hypothetical protein Tsp_08444

gi|339247637

18.9/9.70

62

57

7

0.023

4

deoxyribonuclease II family protein

gi|339241449

38.1/5.95

98

10

2

6.8e-006

5

serine protease

gi|168805931

35.7/5.97

245

18

4

1.1e-020

6

deoxyribonuclease II family protein

gi|339241449

38.1/5.95

382

22

6

2.1e-034

7

deoxyribonuclease II family protein

gi|339241449

38.1/5.95

315

22

6

1.1e-027

8

Not identified

-

-

-

-

-

-

9

deoxyribonuclease II family protein

gi|339241449

38.1/5.95

447

28

7

6.6e-041

10

ps73f07.y1 T. spiralis adult pAMP1 v1 T. spiralis cDNA 5′, mRNA sequence

gi|21410467

22.3/4.83

371

39

9

2e-032

11

ps97f02.y1 T. spiralis adult pAMP1 v1 T. spiralis cDNA 5′, mRNA sequence

gi|21414022

20.7/4.71

369

38

9

3.2e-032

12

PREDICTED: keratin, type II cytoskeletal 1 [Gorilla gorilla gorilla]

gi|160961491

66.0/8.15

232

36

12

1.4e-016

13

serine protease

gi|168805933

48.7/6.33

145

9

2

1.1e-010

14

serine proteinase

gi|13641204

48.7/6.33

116

12

5

8.4e-008

15

deoxyribonuclease II family protein

gi|339241449

38.1/5.95

312

30

9

2.1e-027

16

53 kDa excretory/secretory antigen

gi|805126

4.71/8.42

280

21

8

3.3e-024

17

conserved hypothetical protein

gi|339258426

13.5/4.54

121

19

2

2.6e-008

18

Not identified

-

-

-

-

-

-

19

conserved hypothetical protein

gi|339258426

13.7/4.54

209

29

3

4.2e-017

20

conserved hypothetical protein

gi|339258426

13.5/4.54/

205

42

4

1.1e-016

21

conserved hypothetical protein

gi|316966355

13.5/4.54

92

30

2

2.2e-005

22

conserved hypothetical protein

gi|316966355

13.5/4.54

70

19

2

0.0035

23

conserved hypothetical protein

gi|339258426

13.5/4.54

231

29

3

2.6e-019

24

conserved hypothetical protein

gi|339258426

13.5/4.54

254

59

5

1.3e-021

25

conserved hypothetical protein

gi|339258426

13.54.54

248

59

5

5.3e-021

26

Not identified

-

-

-

-

-

-

27

MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence

gi|13198997

19.9/4.76

345

38

6

8.1e-030

28

MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence

gi|13198997

19.9/4.76

359

38

6

3.2e-031

29

Not identified

-

-

-

-

-

-

30

Not identified

-

-

-

-

-

-

31

MBTsMLA019T7SEQ T. spiralis muscle stage larvae (BC) T. spiralis cDNA clone MBTsMLA019 5′, mRNA sequence

gi|13198997

19.9/4.76

423

55

6

1.3e-037

32

TPAF-aac52c09.g1 T. spiralis _EST T. spiralis cDNA 5′, mRNA sequence

gi|157957485

21.6/9.48

382

57

7

1.6e-033

33

ps19d01.y1 T. spiralis ML CMVsport jasmer T. spiralis cDNA 5′, mRNA sequence

gi|148310851

17.7/10.32

223

53

6

1.3e-017

  1. aTheoretical molecular mass (kDa) and isoelectric point (pI).