IgG subclass responses triggered by r Cs RNASET2. BALB/c mice (n = 6) were immunized s.c. with rCs RNASET2. After 4 (A) and 6 (B) weeks, sera levels of IgG1 and IgG2a specific for rCs RNASET2 were evaluated by ELISA. IgG1 levels were significantly higher than IgG2a at both 4th and 6th week. Error bars represent mean levels ± SD. Statistical significance was analyzed by the Mann–Whitney test (***p < 0.0005).