Figure 1

Gene amplification analyses of transcription profiles of AAEL010714. A) mRNA accumulation profiles in developmental stages and times after adult females feed on blood: fourth instar larvae (L4), white pupae (WP), male pupae (MP), female pupae (FM), newly-emerged males (EM), newly-emerged females (EF), sugar meal adult females (S), and adult females 24, 48 and 72 hPBM. β-actin mRNA levels were used as a control. B) Tissue-specific expression profile. All tissues, midgut (MG), ovaries (OV) and fat bodies (FB), were dissected from Ae. aegypti females at 48 hPBM. The same RNA samples were also analyzed by 18S rRNA-specific primers (Additional file 1 - [25]) as a control. C) Expression profile in female heads dissected at 48 hPBM. β-actin specific primers were used as controls on the same RNA samples. D) Quantification of accumulated of AAEL010714 transcripts by qRT-PCR in adult females 36, 48, 60, 72, 84 and 96 hPBM. All data represent mean values and error bars indicate standard error of the mean; *: p < 0.0001.