Multispacer typing of Rickettsia isolates from humans and ticks in Tunisia revealing new genotypes

Parasites & Vectors

Table 1 Rickettsia species detected in Tunisian patients and in vectors and their spacer genotypes*

N°	Source	Locality	Date	Species	dksA-xerC	rpmE-tRNA^fMET	mppA-purC
P45	Skin biopsy	Hzag	09-12-2009	R. conorii subsp conorii	B^a	KF500414¹	B^b
P73	Skin biopsy	Menzel chaker	09-16-2009	R. conorii subsp israelensis	S^c	C^d	D^e
P75	Skin biopsy	Menzel chaker	09-28-2009	R. conorii subsp israelensis	S	C	D
P77	Skin biopsy	Sidi bouzid	09-30-2009	R. conorii subsp israelensis	S	C	D
F220	C. felis	Kerkennah	30-07-2009	R.felis	AB^f	P^g	-
T116	Rh. sanguineus	Boujarbou	07-22-2009	R. massiliae	AE/CP003319^h	EU250277/CP003319ⁱ	EU250278/CP003319^j
T317	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S^k	C^l	D^m
T322	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S	C	D
T323	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S	C	D
T328	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S	C	D
T330	Rh. sanguineus	Djebeniana	07-22-2009	R. massiliae	-	EU250277/CP003319	EU250278/CP003319
T332	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S	C	D
T336	Rh. sanguineus	Djebeniana	07-22-2009	R. conorii subsp israelensis	S	C	D
T421	Rh. sanguineus	Kerkennah	07-30-2009	R. conorii subsp conorii	Bⁿ	KF539830²	B

Table legend:
*Spacer genotypes are defined according to Fournier et al. database. If the spacer sequence showed a similarity less than 100% to those present in this database, the Genbank accession number of the sequence with 100% similarity or that of the new submitted sequence (1,2) is noted.
-: Amplification or sequencing failed.
Accession Numbers of submitted sequences : ^aKF500412, ^bKF500416, ^cKF500411, ^dKF500413, ^eKF500415, ^fKF539824, ^g:KF539828, ^hKF539823, ⁱKF539827, ^jKF539831, ^kKF539825, ^l:KF539829, ^mKF539832, ⁿKF539826.

ISSN: 1756-3305