Figure 5

Microsporidian infected lymphocytes stained with Giemsa (a and b) and Calcofluor white (c-f). (a) Histological section showing a lymphocyte from a heart infected with numerous microsporidian spores (arrow). (b–f) Kidney imprints; (b and c) Lymphocyte showing at least 10 microsporidian spores inside a lymphocyte. (d) A lymphocyte infected with numerous spores that occupy most of the nucleus; note the size of infected cell compared to erythrocyte (arrow). (e and f) Higher magnification of microsporidian spores inside the nucleus of affected cells. Scale bar: (a)–(d) = 4 μm; (e), (f) = 2 μm.