Nucleospora cyclopteri n. sp., an intranuclear microsporidian infecting wild lumpfish, Cyclopterus lumpus L., in Icelandic waters

Table 1 Results of microsporidian screening of selected organs with nested PCR and Calcofluor white stained histological sections

	Clinical signs	Fish lenght (cm)	Method	Kidney	Heart	Spleen	Skin	Gills	Eggs
Fish 1	None	44	Fluorescence	-ve	-ve	-ve	-ve	-ve	-ve
			PCR 1	-ve	-ve	-ve	-ve	n.s.	-ve
			PCR 2	-ve	+	++	-ve	n.s.	++
Fish 2	Subclinical	41	Fluorescence	*	*	*	n.s.	n.s.	-ve
			PCR 1	+	+	-ve	-ve	n.s.	-ve
			PCR 2	++	++	++	++	n.s.	++
Fish 3	Subclinical	40	Fluorescence	*	*	*	-ve	-ve	-ve
			PCR 1	++	++	+	-ve	n.s.	-ve
			PCR 2	n.a.	n.a.	++	++	n.s.	++
Fish 4	Subclinical	41	Fluorescence	*	*	-ve	-ve	-ve	-ve
			PCR 1	-ve	+	+	+	+	+
			PCR 2	++	++	++	++	++	++
Fish 5	Subclinical	41	Fluorescence	*	*	-ve	-ve	-ve	-ve
			PCR 1	+	-ve	-ve	-ve	+	+
			PCR 2	++	++	+	++	++	++
Fish 6	Macroscopic	48	Fluorescence	**	*	*	*	**	*
			PCR 1	++	++	n.s.	++	-ve	-ve
			PCR 2	n.a	n.a.	n.s.	n.a.	++	++
Fish 7	Macroscopic	42	Fluorescence	*******	**	*******	*	**	*
			PCR 1	++	++	n.s.	++	+	+
			PCR 2	n.a.	n.a.	n.s.	n.a.	++	++
Fish 8	Macroscopic	43	Fluorescence	**	**	**	*	*	*
			PCR 1	++	++	-ve	++	++	++
			PCR 2	n.a.	n.a.	++	n.a.	n.a.	n.a.
Fish 9	Macroscopic	40	Fluorescence	*******	**	*******	*	*******	*
			PCR 1	++	++	++	++	++	-ve
			PCR 2	n.a.	n.a.	n.a.	n.a.	n.a	-ve
Fish 10	Macroscopic	44	Fluorescence	*******	**	*******	*	*******	-ve
			PCR 1	++	++	++	+	n.s.	+
			PCR 2	n.a.	n.a.	n.a.	++	n.s.	++

Five were apparently healthy (fish 1–5) and five had obvious clinical signs (fish 6–10).
Fluorescence: -ve = (−ve) = no spores detected; * = light infection; less than 10 spores or a cluster of spores (inside the same cell) occasionally seen; ** = moderate infection; spores seen in most microscopic fields, their number ranging from 10 to 100; *** = massive infection; more than 100 spores seen in all microscopic fields.
PCR: - ve = No visible band; + = Faint band visible; ++ = Clear to strong band; n.a. = Not applicable (if clearly positive (++) in round 1 the second round was not performed). Other abbreviation: n.s. = No sample.

ISSN: 1756-3305