Effect of TLCK and PMSF on the profile of the proteolytic extracts from Ae. albopictus pupae. Proteolytic activities were detected after incubating the gels for 36 hours at 37°C in Tris–HCl buffer (pH 7.5). Enzymatic assays were performed in the absence of peptidase inhibitors (A), in the presence of 100 μM TLCK (B), and in the presence of 1 mM PMSF (C). The numbers on the left indicate the approximate molecular masses of the peptidases (kDa).