Figure 6
From: Trypsin-like serine peptidase profiles in the egg, larval, and pupal stages of Aedes albopictus
Effect of TLCK and PMSF on the profile of the proteolytic extracts from Ae. albopictus pupae. Proteolytic activities were detected after incubating the gels for 36 hours at 37°C in Tris–HCl buffer (pH 7.5). Enzymatic assays were performed in the absence of peptidase inhibitors (A), in the presence of 100 μM TLCK (B), and in the presence of 1 mM PMSF (C). The numbers on the left indicate the approximate molecular masses of the peptidases (kDa).