Figure 2

Stained histological sections. (A) A section through a gall bladder infected with Sphaeromyxa locodi n. sp. showing large polymorphic plasmodia (thin arrows) occupying large parts of its volume. (B) Mature S. lycodi spores within a plasmodium. (C) Several developing pansporoblasts (arrows) and one mature spore (arrowhead) within a plasmodium. (D) Typical ectoplasm of a plasmodium; a narrow compact outer layer (broad arrow), a triple eosinophilic inner layer, a broader finely granular layer (Gl) enclosed by two radially striated layers (Sl). (E) A less commonly detected ectoplasmic layer with spherical bodies (white arrows) and villar protrusions (black arrow). (A), (C) and (D) are stained with Haematoxylin and Eosin stain but (B) and (E) with Giemsa. Scale bars: (A) = 500 μm; (B) = 30 μm (C) = 10 μm; (D) and (E) = 5μm. Gbw - gall bladder wall. Ep – endoplasm.