Compound DNA vaccine encoding SAG1/ SAG3 with A2/B subunit of cholera toxin as a genetic adjuvant protects BALB/c mice against Toxoplasma gondii

Table 2 CD4 ⁺ CD8 ⁺ subtype of T cells from immunization mice were measured using flow cytometry

Immunization regimen^a	CD4⁺(%)^b	CD8⁺(%)^b	CD4⁺/CD8⁺
PBS	26.71±0.22	18.67±0.92	1.43±0.23
pcDNA3.1	27.62±0.25	24.71±0.85	1.12±0.29
pSAG1	26.45±0.34	26.37±0.98	1.00±0.35
pSAG1/SAG3	24.68±0.14	35.01±0.88	0.70±0.16
pSAG1/SAG3-CTXA2/B	25.15±0.23	38.55±0.94	0.65±0.24

^a Mice were immunized by i.m. route on day 0 and day 14 and day 28 with PBS, pcDNA3.1, pSAG1, pSAG1/SAG3, pSAG1/SAG3-CTXA₂/B.
^b The splenocytes culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITC-labeled anti-mouse CD8⁺ monoclonal antibody and PE-labeled anti-mouse CD4⁺ monoclonal antibody, T lymphocyte subsets were measured using flow cytometry.

ISSN: 1756-3305