Expression of β-glucanase from R. rhodnii Clone 1. Dialyzed lysates and spent media from transformed R. rhodnii was examined for expression of β-glucanase. Expression of β-glucanase was consistently higher in each of the diluted samples, suggesting that there is self-blocking at high protein expression levels. As expected, the expressed protein is found predominantly in the spent media. Bars represents the mean with standard error bars of triplicate samples wells.