Figure 1

A), B) and C) RT-PCR products with TrV primers on 1.5% agarose gel stained with ethidium bromide, at zero, three and forty-five days after inoculation respectively. HL I: 200 bp to 10000 bp; Lane 1: negative control of RNA extraction; 2: IP with PBS; 3: IP with 3 μg empty TrV particles; 4: IP with 3 μg TrV; 5: IP with 0.3 μg TrV; 6: IP with 0,03 μg TrV; 7: Per os with PBS; 8: Per os with 3 μg empty TrV particles; 9: Per os with 3 μg TrV; 10: Negative control of RT-PCR, 11: 12.5 ng of pDNA (TrV). D) RT-PCR products with β-actin primers on 2% agarose gel stained with ethidium bromide forty-five days after inoculation. HL IV: 100 bp to 1000 bp; Lane 1: negative control of RNA extraction; 2: IP with PBS; 3: IP with 3 μg empty TrV particles; 4: IP with 3 μg TrV; 5: IP with 0,3 μg TrV; 6: IP with 0.03 μg TrV; 7: Per os with PBS; 8: Per os with 3 μg empty TrV particles; 9: Per os with 3 μg TrV.