Table 1 PCR primers and conditions
Generic PCR | Primers (5′ -3′) | Cycling structure |
|---|---|---|
JVF (forward) | GTTGATCCTGCCAGTATTATATG | 95°C for 5 min followed by 40 cycles of 95°C for 30 s, 57°C for 30 s, 72 C for 1 min, 72 C for 7 min |
DSPR2 B (reverse) | CACTATTGGAGCTGGAATTAC | |
JVF (forward) | GTTGATCCTGCCAGTATTATATG | 95°C for 5 min followed by 40 cycles of 95°C for 30 s, 50°C for 30 s, 72 C for 1 min, 72 C for 7 min |
EntaRev 390* (reverse) | ATTCCTCGTTATCCGTTAT | |
JVF (forward) | GTTGATCCTGCCAGTATTATATG | 95°C for 5 min followed by 40 cycles of 95°C for 30 s, 55°C for 30 s, 72 C for 1 min, 72 C for 7 min |
EntaRev417* (reverse) | AAAGCTCCTCTCCGATGT |