Validation of a multi-residue method to determine deltamethrin and alpha-cypermethrin in mosquito nets by gas chromatography with electron capture detection (GC-μECD)

Table 3 Analytical data for the low, middle and high dose spiked samples

Spiking level		Alpha-cypermethrin 0.5 g a.i./kg of net		Deltamethrin 0.2 g a.i./kg of net
		Amount (g/kg)	Recovery (%)	Amount (g/kg)	Recovery (%)
Low spiked sample^b n=7	L1	0.424	85.18	0.180	90.33
	L2	0.438	88.39	0.181	91.44
	L3	0.415	84.48	0.176	89.64
	L4	0.428	85.85	0.175	87.95
	L5	0.412	83.75	0.178	90.58
	L6	0.445	89.09	0.181	90.65
	L7	0.419	85.81	0.177	90.72
	L Mean	0.426	86.08	0.178	90.19
	SD	0.01	1.97	0.002	1.12
	RSD %	2.83	2.29	1.317	1.24
	Acceptable mean recovery[12]		80 - 120		80 - 120
	Acceptable RSD[12]		≤ 10		≤ 10
		Alpha-cypermethrin 5 g a.i./kg of net		Deltamethrin 2 g a.i./kg of net
Middle spiked sample n=7	M1	5.230	106.06	2.007	101.90
	M2	5.466	110.38	2.088	105.56
	M3	5.158	104.59	1.953	99.13
	M4	5.290	108.26	2.026	103.79
	M5	5.170	104.20	1.991	100.42
	M6	5.543	112.20	2.117	107.27
	M7	5.109	104.18	1.948	99.41
	M Mean	5.281	107.12	2.019	102.50
	SD	0.16	3.22	0.06	3.14
	RSD %	3.12	3.01	3.19	3.07
	Acceptable mean recovery[12]		90 - 110		90 - 110
	Acceptable RSD[12]		≤ 5		≤ 5
		Alpha-cypermethrin 10 g a.i./kg of net		Deltamethrin 4 g a.i./kg of net
High spiked sample n=6	H1	9.977	101.39	4.141	105.33
	H2	10.034	100.14	4.225	105.54
	H3	10.073	101.17	4.199	105.56
	H4	10.099	102.27	4.470	113.31
	H5	9.802	99.39	4.297	109.06
	H6	9.724	97.79	4.286	107.89
	H Mean	9.951	100.36	4.270	107.78
	SD	0.15	1.61	0.11	3.10
	RSD %	1.55	1.61	2.67	2.88
	Acceptable mean recovery[12]		98 - 102		90 - 110
	Acceptable RSD[12]		≤ 2		≤ 5

^b Active ingredient concentrations in low spiked samples were calculated with reduced calibration curve (0.01, 0.1, 0.5 μg/mL).

ISSN: 1756-3305