Trypanocidal and cytotoxic effects of salinomycin. Bloodstream forms of T. brucei (red squares), HL-60 cells (green circles) and PBMCs (blue triangles) were incubated with varying concentrations of salinomycin. After 72 h of culture, cell viability and proliferation were determined with the colorimetric dye resazurin. Mean values ± SD of three (PBMCs) or four (T. brucei and HL-60) experiments are shown. Dose–response curves were calculated from mean values using the 4-parameter logistic model.