Skip to main content

Table 1 Real-time PCR for T. gondii in muscle and brain tissue samples (tested in duplicate [a, b]) from 18 seropositive (IFAT, IHA, PrioCHECK ® Toxoplasma Ab SR ELISA) naturally exposed sheep (Group 2)

From: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep

Sheep No.

Tissue

PCR result

ELISA PP value

IHA result

IFAT titer

 

Brain

Skeletal muscle

    
 

PCR a (CTvalue)

PCR b (CTvalue)

PCR a (CTvalue)

PCR b (CTvalue)

    

01-1306

+ (34.76)

+ (36.30)

+ (32.16)

+ (32.80)

+

80.45

+

1:640

02-1308

-

-

-

-

-

36.17

+

1:160

03-1503

-

-

-

-

-

104.73

+

1:1280

04-1628

+ (32.80)

+ (33.96)

-

-

+

105.26

+

1:1280

05-1640

-

-

-

-

-

85.89

+

1:2560

06-1641

-

-

+ (29.38)

+ (29.16)

+

79.83

+

1:640

07-1646

+ (28.48)

+ (28.89)

-

-

+

73.91

+

1:1280

08-1649

-

-

-

-

-

38.26

+

1:1280

09-1651

-

-

-

-

-

85.46

+

1:1280

10-1653

+ (30.67)

+ (30.50)

-

-

+

95.74

+

1:2560

11-3879

-

-

+ (36.25)

+ (38.49)

+

85.32

+

≥1:5120

12-3880

-

-

+ (35.15)

+ (32.69)

+

91.87

+

≥1:5120

13-3883

-

-

-

-

-

74.31

+

1:2560

14-3885

-

-

+ (34.78)

+ (38.76)

+

47.41

+

1:640

15-3886

-

-

-

-

-

38.19

+

1:640

16-4947

-

+ (38.83)

-

-

+

101.33

+

≥1:5120

17-4948

-

-

+ (36.82)

+ (38.69)

+

79.08

+

≥1:5120

18-5785

+ (26.34)

+ (26.57)

+ (36.77)

+ (37.57)

+

26.32

+

1:1280

Total positive

6/18

7/18

11/18

18/18

18/18

18/18

  1. Cut-offs considered: IFAT: 1:40; IHA: 1:80; ELISA PP 20. C T : Cycle threshold. (Real-time PCR results from seronegative sheep not displayed).