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Table 1 Real-time PCR for T. gondii in muscle and brain tissue samples (tested in duplicate [a, b]) from 18 seropositive (IFAT, IHA, PrioCHECK ® Toxoplasma Ab SR ELISA) naturally exposed sheep (Group 2)

From: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep

Sheep No. Tissue PCR result ELISA PP value IHA result IFAT titer
  Brain Skeletal muscle     
  PCR a (CTvalue) PCR b (CTvalue) PCR a (CTvalue) PCR b (CTvalue)     
01-1306 + (34.76) + (36.30) + (32.16) + (32.80) + 80.45 + 1:640
02-1308 - - - - - 36.17 + 1:160
03-1503 - - - - - 104.73 + 1:1280
04-1628 + (32.80) + (33.96) - - + 105.26 + 1:1280
05-1640 - - - - - 85.89 + 1:2560
06-1641 - - + (29.38) + (29.16) + 79.83 + 1:640
07-1646 + (28.48) + (28.89) - - + 73.91 + 1:1280
08-1649 - - - - - 38.26 + 1:1280
09-1651 - - - - - 85.46 + 1:1280
10-1653 + (30.67) + (30.50) - - + 95.74 + 1:2560
11-3879 - - + (36.25) + (38.49) + 85.32 + ≥1:5120
12-3880 - - + (35.15) + (32.69) + 91.87 + ≥1:5120
13-3883 - - - - - 74.31 + 1:2560
14-3885 - - + (34.78) + (38.76) + 47.41 + 1:640
15-3886 - - - - - 38.19 + 1:640
16-4947 - + (38.83) - - + 101.33 + ≥1:5120
17-4948 - - + (36.82) + (38.69) + 79.08 + ≥1:5120
18-5785 + (26.34) + (26.57) + (36.77) + (37.57) + 26.32 + 1:1280
Total positive 6/18 7/18 11/18 18/18 18/18 18/18
  1. Cut-offs considered: IFAT: 1:40; IHA: 1:80; ELISA PP 20. C T : Cycle threshold. (Real-time PCR results from seronegative sheep not displayed).