Sheep No.
|
Tissue
|
PCR result
|
ELISA PP value
|
IHA result
|
IFAT titer
|
---|
|
Brain
|
Skeletal muscle
| | | | |
---|
|
PCR a (CTvalue)
|
PCR b (CTvalue)
|
PCR a (CTvalue)
|
PCR b (CTvalue)
| | | | |
---|
01-1306
|
+ (34.76)
|
+ (36.30)
|
+ (32.16)
|
+ (32.80)
|
+
|
80.45
|
+
|
1:640
|
02-1308
|
-
|
-
|
-
|
-
|
-
|
36.17
|
+
|
1:160
|
03-1503
|
-
|
-
|
-
|
-
|
-
|
104.73
|
+
|
1:1280
|
04-1628
|
+ (32.80)
|
+ (33.96)
|
-
|
-
|
+
|
105.26
|
+
|
1:1280
|
05-1640
|
-
|
-
|
-
|
-
|
-
|
85.89
|
+
|
1:2560
|
06-1641
|
-
|
-
|
+ (29.38)
|
+ (29.16)
|
+
|
79.83
|
+
|
1:640
|
07-1646
|
+ (28.48)
|
+ (28.89)
|
-
|
-
|
+
|
73.91
|
+
|
1:1280
|
08-1649
|
-
|
-
|
-
|
-
|
-
|
38.26
|
+
|
1:1280
|
09-1651
|
-
|
-
|
-
|
-
|
-
|
85.46
|
+
|
1:1280
|
10-1653
|
+ (30.67)
|
+ (30.50)
|
-
|
-
|
+
|
95.74
|
+
|
1:2560
|
11-3879
|
-
|
-
|
+ (36.25)
|
+ (38.49)
|
+
|
85.32
|
+
|
≥1:5120
|
12-3880
|
-
|
-
|
+ (35.15)
|
+ (32.69)
|
+
|
91.87
|
+
|
≥1:5120
|
13-3883
|
-
|
-
|
-
|
-
|
-
|
74.31
|
+
|
1:2560
|
14-3885
|
-
|
-
|
+ (34.78)
|
+ (38.76)
|
+
|
47.41
|
+
|
1:640
|
15-3886
|
-
|
-
|
-
|
-
|
-
|
38.19
|
+
|
1:640
|
16-4947
|
-
|
+ (38.83)
|
-
|
-
|
+
|
101.33
|
+
|
≥1:5120
|
17-4948
|
-
|
-
|
+ (36.82)
|
+ (38.69)
|
+
|
79.08
|
+
|
≥1:5120
|
18-5785
|
+ (26.34)
|
+ (26.57)
|
+ (36.77)
|
+ (37.57)
|
+
|
26.32
|
+
|
1:1280
|
Total positive
|
6/18
|
7/18
|
11/18
|
18/18
|
18/18
|
18/18
|
- Cut-offs considered: IFAT: 1:40; IHA: 1:80; ELISA PP 20. C
T
: Cycle threshold. (Real-time PCR results from seronegative sheep not displayed).