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Table 2 Comparison of PrioCHECK ® Toxoplasma Ab SR ELISA-results (cut-off PP 20) from 96 sheep from Switzerland (Group 2) to results obtained by IFAT, IHA (not performed with meat juice samples) and real-time PCR, and inter-rater agreement (kappa value)

From: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep

   IFAT serum cut-off 1:40 IFAT serum cut-off 1:80 IHA serum cut-off 1:80 Real-time PCR brain/muscle
A   positive negative total positive negative total positive negative total positive negative total
ELISA serum positive 18 0 18 18 0 18 18 0 18 11 7 18
  negative 0 78 78 0 78 78 0 78 78 0 78 78
  total 18 78 96 18 78 96 18 78 96 11 85 96
  Kappa 1.0    1.0    1.0    0.719   
  Standard Error 0    0    0    0.0983   
  95% CI 1.0-1.0    1.0-1.0    1.0-1.0    0.526-0.911   
    IFAT meat juice cut-off 1:40 IFAT meat juice cut-off 1:80 Real-time PCR brain/muscle   
B    positive negative total positive negative total positive negative total   
ELISA meat juice 1:10 positive   18 2 20 15 5 20 11 9 20   
  negative   0 76 76 0 76 76 0 76 76   
  total   18 78 96 15 81 96 11 85 96   
  Kappa   0.934    0.826    0.659     
  Standard Error   0.0458    0.0746    0.102     
  95% CI   0.845-1    0.68-0.972   0.46-0.859      
ELISA meat juice 1:20 positive   17 1 18 15 3 18 11 7 18   
  negative   1 77 78 0 78 78 0 78 78   
  total   18 78 96 15 81 96 11 85 96   
  Kappa   0.932    0.890    0.719     
  Standard Error   0.0478    0.0619    0.0983     
  95% CI   0.838-1    0.769-1    0.526-0.911     
  1. A: serum samples; B: meat juice samples at a dilution of 1:10 and 1:20. CI: confidence interval.