Table 3 Comparative results of seven sheep with recent natural T. gondii infection; serum tested by PrioCHECK ® Toxoplasma Ab SR ELISA (cut-off PP 20), IFAT (cut-off 1:40) and IHA (cut-off 1:80) at day 0, 25 and 35 (slaughter) and real-time PCR of brain and skeletal muscle at day 35
Sheep no. | Time course (days) | ELISA (PP)* | IFAT (titer) | IHA | Real-time PCR brain | Real-time PCR skeletal muscle |
|---|---|---|---|---|---|---|
4947 | 0 | - (3.69) | - (<1:40) | - | ||
25 | + (89.02) | + (≥1:5,120) | + | |||
35 | + (101.33) | + (≥1:5,120) | + | + | - | |
4948 | 0 | - (3.28) | - (<1:40) | - | ||
25 | + (82.43) | + (≥1:5,120) | + | |||
35 | + (79.08) | + (≥1:5,120) | + | - | + | |
3879 | 0 | - (4.24) | + (1:40) | + | ||
25 | + (78.86) | + (≥1:5,120) | + | |||
35 | + (85.32) | + (≥1:5,120) | + | - | + | |
3880 | 0 | - (6.20) | + (1:80) | + | ||
25 | + (86.23) | + (≥1:5,120) | + | |||
35 | + (91.87) | + (≥1:5,120) | + | - | + | |
3883 | 0 | - (9.07) | + (1:40) | - | ||
25 | + (38.32) | + (≥1:5,120) | + | |||
35 | + (74.31) | + (1:2,560) | + | - | - | |
3885 | 0 | - (5.88) | - (<1:40) | - | ||
25 | + (44.29) | + (1:1,280) | + | |||
35 | + (47.41) | + (1:640) | + | - | + | |
3886 | 0 | - (12.39) | - (<1:40) | - | ||
25 | + (31.39) | + (1:1,280) | + | |||
35 | + (38.19) | + (1:640) | + | - | - |