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Table 3 Comparative results of seven sheep with recent natural T. gondii infection; serum tested by PrioCHECK ® Toxoplasma Ab SR ELISA (cut-off PP 20), IFAT (cut-off 1:40) and IHA (cut-off 1:80) at day 0, 25 and 35 (slaughter) and real-time PCR of brain and skeletal muscle at day 35

From: Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep

Sheep no. Time course (days) ELISA (PP)* IFAT (titer) IHA Real-time PCR brain Real-time PCR skeletal muscle
4947 0 - (3.69) - (<1:40) -   
  25 + (89.02) + (≥1:5,120) +   
  35 + (101.33) + (≥1:5,120) + + -
4948 0 - (3.28) - (<1:40) -   
  25 + (82.43) + (≥1:5,120) +   
  35 + (79.08) + (≥1:5,120) + - +
3879 0 - (4.24) + (1:40) +   
  25 + (78.86) + (≥1:5,120) +   
  35 + (85.32) + (≥1:5,120) + - +
3880 0 - (6.20) + (1:80) +   
  25 + (86.23) + (≥1:5,120) +   
  35 + (91.87) + (≥1:5,120) + - +
3883 0 - (9.07) + (1:40) -   
  25 + (38.32) + (≥1:5,120) +   
  35 + (74.31) + (1:2,560) + - -
3885 0 - (5.88) - (<1:40) -   
  25 + (44.29) + (1:1,280) +   
  35 + (47.41) + (1:640) + - +
3886 0 - (12.39) - (<1:40) -   
  25 + (31.39) + (1:1,280) +   
  35 + (38.19) + (1:640) + - -
  1. * PP: Index for percentage of positivity.