| | | |
PrioCHECK® Toxoplasma Ab SR ELISA
|
|---|
|
Reference test
|
Sample
|
AUC/P*
|
Selected cut-off in ELISA
|
% relative sensitivity (95% confidence interval)
|
% relative specificity (95% confidence interval)
|
|---|
|
A
|
|
IFAT (cut-off 1:40)
|
serum
|
1.000/<0.000
|
19.79
|
100 (81.5 - 100.0)
|
100 (95.4 – 100.0)
|
|
IFAT (cut-off 1:80)
|
serum
|
1.000/<0.000
|
19.79
|
100 (81.5 - 100.0)
|
100 (95.4 – 100.0)
|
|
IHA (cut-off 1:80)
|
serum
|
1.000/<0.000
|
19.79
|
100 (81.5 - 100.0)
|
100 (95.4 – 100.0)
|
|
Real-time PCR
|
brain/muscle
|
0.959/<0.0001
|
19.79
|
100 (71.5 - 100.0) §
|
91.76 (83.8 - 96.6)§
|
|
IFAT (cut-off 1:40)
|
meat juice 1:10**
|
0.999/<0.0001
|
20.29
|
100 (81.5 - 100.0)
|
98.72 (93.1 - 100.0)
|
|
IFAT (cut-off 1:80)
|
meat juice 1:10**
|
0.996/<0.0001
|
27.64
|
100 (78.2 - 100.0)
|
97.53 (91.4 - 99.7)
|
|
IFAT (cut-off 1:40)
|
meat juice 1:20**
|
0.999/<0.0001
|
15.83
|
100 (81.5 - 100.0)
|
98.72 (93.1 - 100.0)
|
|
IFAT (cut-off 1:80)
|
meat juice 1:20**
|
0.998/<0.0001
|
27.45
|
100 (78.2 - 100.0)
|
98.77 (93.3 - 100.0)
|
|
B
| | | | | |
|
IFAT (cut-off 1:40)
|
serum
|
0.991/<0.0001
|
17.24
|
93.33 (88.1 - 96.8)
|
98.67 (95.3 - 99.8)
|
|
IFAT (cut-off 1:80)
|
serum
|
0.985/<0.0001
|
21.05
|
96.43 (91.9 - 98.8)
|
96.87 (92.9 - 99.0)
|
|
IHA (cut-off 1:80)
|
serum
|
0.998/<0.0001
|
21.05
|
97.87 (93.9 - 99.6)
|
98.74 (95.5 - 99.8)
|
- A: Tests performed with samples from Swiss sheep (Group 2), B: Tests performed with samples from Austrian sheep (Groups 3 and 4).
- *Significance level P (Area = 0.5); ** meat juice dilution; AUC: Area under curve.
- § Note: The apparent low specificity of ELISA when real-time PCR is taken as reference test might be related to the low diagnostic sensitivity of PCR techniques linked to the small size of the analyzed sample. All real-time PCR positive animals were recognized as positive by ELISA (100% relative sensitivity), but some serologically positive animals in ELISA had negative PCR results accounting for a (potentially erroneous) relative lower specificity of the ELISA.
