Table 2 Differentially expressed proteins between T. gondii -infected and non-infected mouse brain tissues

Day 7, down-regulated proteins

1

IPI00230394

Lamin-B1

288

9.00E-25

27

36

66973

5.11

ASAPATPLSPTR;LQEKEELR;SLETENSALQLQVTEREEVR;ALYETELADARR;LREYEAALNSK;VDLENRCQSLTEDLEFRKNMYEEEINETRR;LVEVDSGR;LAQALHEMREQHDAQVRLYKEELEQTYHAK;LALDMEISAYR;LKNTSEQDQPMGGWEMIR;NQNSWGTGEDVK;NSQGEEVAQR;TTIPEEEEEEEEEPIGVAVEEERFHQQGAPR

−2.91

Structural molecule activity

2

IPI00944143

Serpin B6 isoform a

218

9.00E-18

9

25

45201

5.99

SRPGCCAGPRGYR;NVFLSPMSISSALAMVFMGAK;TGTQYLLR;FYEAELEELDFQGATEESR;FIEWTR;LGMTDAFGGR;AFVEVNEEGTEAAAATAGMMTVR

−1.68

Enzyme regulator activity and metabolic process

3

IPI00110721

Isoform 1 of Glyoxalase domain-containing protein 4

508

9.00E-47

12

41

33581

5.28

FQTVHFFRDVLGMQVLRHEEFEEGCK;LGNDFMGITLASSQAVSNAR;VAEGIFETEAPGGYKFYLQDR;IYEQDEEKQR;LELQGIQGAVDHAAAFGR;ELPDLEDLMKR;LLDDAMEADKSDEWFATR

−1.51

Cell part and organelle

4

IPI00113660

Proteasome activator complex subunit 3

471

4.50E-43

9

42

29602

5.69

ITSEAEDLVANFFPK;RLDECEEAFQGTK;SNQQLVDIIEKVKPEIR;IEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITR;YPHVEDYRRTVTEIDEKEYISLR

−1.70

Enzyme regulator activity

5

IPI00130344

Chloride intracellular channel protein 1

202

3.60E-16

6

39

27338

5.09

IGNCPFSQR;YPKLAALNPESNTSGLDIFAK;VLDNYLTSPLPEEVDETSAEDEGISQR;GFTIPEAFR;YLSNAYAREEFASTCPDDEEIELAYEQVAR

−1.89

Transporter activity

6

IPI00121851

Prefoldin subunit 3

133

2.90E-09

4

15

22592

6

FLLADNLYCK;NLDSLEEDLDFLR;VYNWDVKR

−1.51

Metabolic process

7

IPI00315794

Cytochrome b5

212

3.60E-17

6

54

16365

4.79

VEGSEPSVTYYR;NSAEETWMVIHGRVYDITRFLSEHPGGEEVLLEQAGADATESFEDVGHSPDAR;QYYIGDVHPSDLKPK

−1.70

Binding and enzyme activator activity

8

IPI00124501

Galectin-related protein A

257

1.10E-21

8

57

19172

5.12

LDDGHLNNSLGSPVQADVYFPRLIVPFCGHIK;AVFTDR;NSCISGERGEEQSAIPYFPFIPDQPFRVEILCEHPR;VFVDGHQLFDFYHRIQTLSAIDTIK

−1.74

Binding

9

IPI00308984

Eukaryotic translation initiation factor 1A

248

9.00E-21

3

28

16564

5.07

ELVFKEDGQEYAQVIK;LEAMCFDGVKR;VWINTSDIILVGLR

−1.67

Binding

Day 7, up-regulated proteins

10

IPI00131830

Serine protease inhibitor A3K

470

5.70E-43

9

28

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR

2.55

Enzyme regulator activity

11

IPI00131830

Serine protease inhibitor A3K

511

4.50E-47

12

35

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETK;AVLDVAETGTEAAAATGVIGGIR

3.15

Enzyme regulator activity

12

IPI00131830

Serine protease inhibitor A3K

463

2.90E-42

10

28

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR

6.30

Enzyme regulator activity

13

IPI00131830

Serine protease inhibitor A3K

564

2.30E-52

14

35

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETKK;AVLDVAETGTEAAAATGVIGGIR

4.02

Enzyme regulator activity

14

IPI00131830

Serine protease inhibitor A3K

596

1.40E-55

12

35

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ELISELDER;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYRLEEDVLPEMGIKEVFTEQADLSGITETK;AVLDVAETGTEAAAATGVIGGIR

5.80

Enzyme regulator activity

15

IPI00310972

GTP-GDP dissociation stimulator 1 isoform b

490

5.70E-45

18

27

61381

5.28

NEFMR;DQEVLLQTGR;EQFASTNIAEELVK;QIEHDKREMIFEVLAPLAENDAIK;LMDLLDRHVEDGNVTVQHAALSALR;SEMPPVQFK;MLIDAQAEAAEQLGK;LVEWCEAKDHAGVMGESNR;DLASAQLVQILHRLLADER;SVAQQASLTEQR

8.32

Cell part and organelle part

Day 14, down-regulated proteins

16

IPI00131871

COP9 signalosome complex subunit 4

449

7.20E-41

18

50

46541

5.57

QLLTDFCTHLPNLPDSTAK;VISFEEQVASIRQHLASIYEKEEDWRNAAQVLVGIPLETGQKQYNVDYKLETYLK;LYLEDDDPVQAEAYINRASLLQNESTNEQLQIHYK;KFIEAAQR;TIVHESERLEALKHALHCTILASAGQQR;MLATLFKDERCQQLAAYGILEK;ATTADGSSILDR;IASQMITEGRMNGFIDQIDGIVHFETR

−2.50

Metabolic process

17

IPI00338039

Tubulin beta-2A chain

591

4.50E-55

22

42

50274

4.78

MREIVHIQAGQCGNQIGAKFWEVISDEHGIDPTGSYHGDSDLQLERINVYYNEAAGNK;AILVDLEPGTMDSVR;GHYTEGAELVDSVLDVVRK;IREEYPDRIMNTFSVMPSPK;FPGQLNADLRKLAVNMVPFPRLHFFMPGFAPLTSRGSQQYRALTVPELTQQMFDSKNMMAACDPR;ISEQFTAMFRR

−1.90

Structural molecule activity

18

IPI00113660

Proteasome activator complex subunit 3

450

5.70E-41

11

43

29602

5.69

ERITSEAEDLVANFFPK;RLDECEEAFQGTK;SNQQLVDIIEKVKPEIR;IEDGNNFGVSIQEETVAELRTVESEAASYLDQISRYYITR;YPHVEDYRRTVTEIDEKEYISLR

−2.04

Enzyme regulator activity

19

IPI00407019

Tetratricopeptide repeat protein 19

348

9.00E-31

12

23

41550

5.87

LSIMKDEPEAAELILHDALRLAYESDNRK;GQLENAEQLFK;QLSQAQR;ALQICQEIQGER;EIYQEALKR;RDEVSVQHIREELAELSR

−1.99

Cell part, organelle and organelle part

20

IPI00133440

Prohibitin

688

9.00E-65

14

42

29859

5.57

AVIFDRFR;DLQNVNITLRILFRPVASQLPRIYTSIGEDYDERVLPSITTEILK;FDAGELITQR;QVSDDLTER;QVAQQEAER;AAELIANSLATAGDGLIELRKLEAAEDIAYQLSR

−4.73

Metabolic process

21

IPI00313962

Ubiquitin carboxyl-terminal hydrolase isozyme L1

340

5.70E-30

8

39

25165

5.14

LGVAGQWR;QIEELKGQEVSPK;QFLSETEKLSPEDR;CFEKNEAIQAAHDSVAQEGQCR;MPFPVNHGASSEDSLLQDAAK;EFTEREQGEVR

−1.68

Cell proliferation

22

IPI00322312

Rho GDP-dissociation inhibitor 1

536

1.40E-49

12

49

23450

5.12

SIQEIQELDKDDESLR;YKEALLGRVAVSADPNVPNVIVTR;EGVEYR;YIQHTYR;IDKTDYMVGSYGPRAEEYEFLTPMEEAPK;FTDDDKTDHLSWEWNLTIK

−4.10

Enzyme regulator activity

23

IPI00845712

Isoform 1 of UPF0424 protein C1orf128 homolog

540

5.70E-50

17

73

24405

5.48

CAAEREEPPEQRGLAYGLYLR;LQCLNESR;GVFKPWEER;FVESDADEELLFNIPFTGNVKLKGVIIMGEDDDSHPSEMR;NIPQMSFDDTEREPEQTFSLNRDITGELEYATK;IFYIGLRGEWTELRRHEVTICNYEASANPADHRVHQVTPQTHFIS;

−2.40

Cell part, organelle and organelle part

24

IPI00221826

Isoform Short of Splicing factor, arginine/serine-rich 3

226

1.40E-18

7

41

14422

10.12

DSCPLDCK;AFGYYGPLRSVWVARNPPGFAFVEFEDPR;NRGPPPSWGR;DDYRR

−1000000

Metabolic process

Day 14, up-regulated proteins

25

IPI00123613

Protein kinase C and casein kinase substrate in neurons protein 1

382

3.60E-34

15

40

50886

5.15

LCNDLMSCVQER;AYAQQLTDWAKR;AWGAMMTEADKVSELHQEVK;AYHLACKEER;TTPQYMEGMEQVFEQCQQFEEKR;HLNLAENSSYMHVYRELEQAIRGADAQEDLR;KAEGATLSNATGAVESTSQAGDR;ALYDYDGQEQDELSFK;LGEEDEQGWCRGRLDSGQLGLYPANYVEAI

4.65

Catalytic activity and binding

26

IPI00110265

Isoform 1 of NAD-dependent deacetylase sirtuin-2

255

1.80E-21

11

35

43856

5.23

NLFTQTLGLGSQK;LLDELTLEGVTRYMQSER;VICLVGAGISTSAGIPDFR;HPEPFFALAK;CYTQNIDTLER;IFSEATPRCEQCQSVVKPDIVFFGENLPSR;APLATPR;ELEDLVRREHANIDAQSGSQAPNPSTTISPGK

1.57

Catalytic activity and binding

27

IPI00649438

Novel protein similar to splicing factor, arginine/serine-rich 3

80

0.00061

3

19

19404

11.41

NPPGFAFVEFEDPRDAADAVR;NRGLPPSWGR

1.65

Metabolic process

Day 21, down-regulated proteins

28

IPI00133522

Protein disulfide-isomerase

408

9.00E-37

17

37

57507

4.79

SNFEEALAAHK;ALAPEYAKR;LKAEGSEIR;VDATEESDLAQQYGVR;YQLDKDGVVLFKKFDEGRNNFEGEITK;HNQLPLVIEFTEQTAPK;ILFIFIDSDHTDNQR;KEECPAVR;YKPESDELTAEKITEFCHR;IKPHLMSQEVPEDWDKQPVKVLVGANFEEVAFDEKK;LGETYKDHENIIIAK;FFPASADR

−1.52

Metabolic process

29

IPI00756786

SH3-domain GRB2-like endophilin B2

258

9.00E-22

11

26

45165

5.44

KLASDAGIFFTR;FGQAEKTELDAHFENLLAR;NWTER;QTEVLLQPNPSAR;ATCEGDTVPDFQETRPR;VAQTEFDRQAEVTRLLLEGISSTHVNHLR;SQTTYYAQCYR

−1.50

Cellular process

30

IPI00111556

Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

239

7.20E-20

10

34

36123

5.21

SPDTNYLFMGDYVDR;QITQVYGFYDECLRKYGNANVWK;LQEVPHEGPMCDLLWSDPDDRGGWGISPR;AHQLVMEGYNWCHDRNVVTIFSAPNYCYR;YSFLQFDPAPR

−2.10

Metabolic process

31

IPI00798544

Isoform 2 of Enolase-phosphatase E1

384

2.30E-34

11

24

25457

4.92

QLQGHMWK;MKAEFFADVVPAVRRWREAGMKVYIYSSGSVEAQK;GHKVDSESYRK

−3.54

Metabolic process

32

IPI00230145

Ferritin heavy chain

408

9.00E-37

11

65

21224

5.53

QNYHQDAEAAINR;YFLHQSHEEREHAEK;KPDRDDWESGLNAMECALHLEKSVNQSLLELHKLATDKNDPHLCDFIETYYLSEQVK;ELGDHVTNLRKMGAPEAGMAEYLFDKHTLGHGDES

−1.56

Immune system process

Day 21, up-regulated proteins

33

IPI00403810

Tubulin alpha-1C chain

174

2.30E-13

5

16

50562

4.96

AVFVDLEPTVIDEVR;EIIDLVLDR;NLDIERPTYTNLNR;FDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK

1.56

Structural molecule activity

34

IPI00221540

Erlin-2

339

7.20E-30

8

17

38077

5.37

SVQTTLQTDEVK;VTKPNIPEAIRR;VAQVAEITYGQK;KISEIEDAAFLAR;AKADAECYTALK

1.85

Metabolic process

35

IPI00230192

Isoform Alpha-1 of Guanine nucleotide-binding protein G(o) subunit alpha

170

5.70E-13

5

16

40629

5.34

AMDTLGVEYGDKER;LWGDSGIQECFNR;YYLDSLDRIGAGDYQPTEQDILR;LFDVGGQR

1.54

Signal transducer activity

36

IPI00653664

Isoform 1 of Enolase-phosphatase E1

365

1.80E-32

11

27

28696

4.79

EYLQTHWEEEECQQDVSLLR;QLQGHMWK;MKAEFFADVVPAVRRWREAGMKVYIYSSGSVEAQK;VDSESYRK

1.96

Metabolic process

37

IPI00130280

ATP synthase subunit alpha

376

1.40E-33

11

18

59830

9.22

TGTAEMSSILEERILGADTSVDLEETGRVLSIGDGIAR;NVQAEEMVEFSSGLK;TGAIVDVPVGEELLGR;APGIIPRISVREPMQTGIKAVDSLVPIGR;ELI IGDR

2.43

Transporter activity and developmental process

38

IPI00129161

Somatotropin

201

4.50E-16

14

45

24986

5.97

AQHLHQLAADTYKEFERAYIPEGQR;EEAQQRTDMELLR;IFTNSLMFGTSDR;LKDLEEGIQALMQELEDGSPR;QTYDKFDANMR;AETYLR;RFVESSCAF

19.73

Cell proliferation

Spots 39–46 were down-regulated on both day 14 and day 21

39

IPI00119113

V-type proton ATPase subunit B

641

4.50E-60

34

64

56857

5.57

MALRAMRGIVNGAAPELPVPTGGPMAGAR;NYLSQPR;YAEIVHLTLPDGTKR;AVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGR;GPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIAR;IPIFSAAGLPHNEIAAQICR;SKDVVDYSEENFAIVFAAMGVNMETAR;LALTTAEFLAYQCEKHVLVILTDMSSYAEALR;RGFPGYMYTDLATIYER;QIYPPINVLPSLSRLMKSAIGEGMTR;DHADVSNQLYACYAIGK;AVVGEEALTSDDLLYLEFLQK;NFITQGPYENRTVYETLDIGWQLLR;RIPQSTLSEFYPR

−2.11

Transporter activity

40

IPI00759870

Isoform 4 of Heterogeneous nuclear ribonucleoproteins C1/C2

201

4.50E-16

3

9

32261

4.95

GFAFVQYVNER;MYSYPARVPPPPPIAR

−1.52

Metabolic process

41

IPI00115117

Stomatin-like protein 2

231

4.50E-19

12

32

38475

8.95

NTVILFVPQQEAWVVER;ILEPGLNVLIPVLDR;ASYGVEDPEYAVTQLAQTTMR;YEIKDIHVPPRVKESMQMQVEAER;ATVLESEGTR;APVPGAQNSSQSRRDVQATDTSIEELGR

−2.33

Organelle and organelle part

42

IPI00830393

Putative uncharacterized protein Cops6

209

7.20E-17

8

31

33855

5.65

SQEGRPMQVIGALIGK;NIEVMNSFELLSHTVEEK;QVCEIIESPLFLK;IGVDHVAR;LILEYVKASEAGEVPFNHEILR;TCNTMNQFVNKFNVLYDR

−2.91

Cellular process and metabolic process

43

IPI00311515

Beta-soluble NSF attachment protein

415

1.80E-37

20

69

33878

5.32

EAVQLMAEAEKR;ASHSFLRGLFGGNTRIEEACEMYTR;NWSAAGNAFCQAAK;HDSATSFVDAGNAYK;HHITIAEIYETELVDIEKAIAHYEQSADYYKGEESNSSANK;VAAYAAQLEQYQKAIEIYEQVGANTMDNPLLK;AALCHFIVDELNAKLALEKYEEMFPAFTDSRLLEAHEEQNSEAYTEAVKEFDSISRLDQWLTTMLLR;

−1.67

Cellular process

44

IPI00322312

Rho GDP-dissociation inhibitor 1

396

1.40E-35

9

42

23450

5.12

SIQEIQELDKDDESLR;YKEALLGRVAVSADPNVPNVIVTR;EGVEYR;YIQHTYR;IDKTDYMVGSYGPR;FTDDDKTDHLSWEWNLTIK

−1000000

Enzyme regulator activity

45

IPI00133853

UPF0687 protein C20orf27 homolog

268

9.00E-23

8

50

19692

6.19

FAAGHDAEGSQSHVHFDEK;YEITFTLPPVR;ETPVHSLHLKLLSVTPTSEGYSIK;EGVLKEEMLLACEGDIGTCVR;HHGTPMLLDGVK

−1.70

Cell part and organelle part

46

IPI00315504

ADP-ribosylation factor-like protein 2

75

0.0016

2

8

21022

5.67

ELQSLLVEER;SHHWR

−1.66

Binding

Spots 47–56 were up-regulated on both day 14 and day 21

47

IPI00129526

Endoplasmin

766

1.40E-72

33

38

92703

4.74

TDDEVVQREEEAIQLDGLNASQIR;FAFQAEVNR;NKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVK;NLLHVTDTGVGMTREELVKEVEEDEYKAFYK;ESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKK;RVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSR;IADEKYNDTFWK;LGVIEDHSNR;FQSSHHSTDITSLDQYVER;QDKIYFMAGSSRKEAESSPFVEREATEKEFEPLLNWMK;LTESPCALVASQYGWSGNMER;DISTNYYASQKKTFEINPR;AYGDRIER

1.54

Metabolic process

48

IPI00123639

Calreticulin

292

3.60E-25

5

16

48136

4.33

EQFLDGDAWTNR;HEQNIDCGGGYVK;IDNSQVESGSLEDDWDFLPPKKIKDPDAAKPEDWDER;GEWKPR

1.66

Immune system process,

49

IPI00131830

Serine protease inhibitor A3K

175

1.80E-13

6

21

47021

5.05

ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;AVLDVAETGTEAAAATGVIGGIR;

1000000

Enzyme regulator activity

50

IPI00131830

Serine protease inhibitor A3K

336

1.40E-29

9

27

47021

5.05

GKTMEEILEGLK;DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;AVLDVAETGTEAAAATGVIGGIR

12.57

Enzyme regulator activity

51

IPI00131830

Serine protease inhibitor A3K

316

1.40E-27

8

26

47021

5.05

DLQILAEFHEK;ALYQTEAFTADFQQPTEAK;ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLRK;FSIASNYR;AVLDVAETGTEAAAATGVIGGIR

5.29

Enzyme regulator activity

52

IPI00131830

Serine protease inhibitor A3K

187

1.10E-14

5

16

47021

5.05

ISFDPQDTFESEFYLDEKR;HFRDEELSCSVLELK;MQQVEASLQPETLR;AVLDVAETGTEAAAATGVIGGIR

1000000

Enzyme regulator activity

53

IPI00131287

Isoform 1 of N-terminal EF-hand calcium-binding protein 2

374

2.30E-33

11

28

43870

5.18

APLVPDIPSADPGPGPAASRGGTAVILDIFR;KVYEGGSNVDQFVTR;YGGPTPPYIPNHK;TLSFDLQQRLSDEEGTNMHLQLVRQEMAVCPEQLSEFLDSLR;NCFHVAAVR

1000000

Cell part and binding

54

IPI00928020

Putative uncharacterized protein Otub1

331

4.50E-29

7

25

28190

5.15

IQQEIAVQNPLVSER;EYAEDDNIYQQK;YSYIRKTRPDGNCFYR;LLTSGYLQR;FFEHFIEGGR

1.89

Metabolic process

55

IPI00323571

Apolipoprotein E

329

7.20E-29

14

39

35901

5.56

FWDYLR;ELEEQLGPVAEETR;LGADMEDLR;NEVHTMLGQSTEEIR;LSTHLR;LGPLVEQGR;TANLGAGAAQPLR;AQAFGDR;GRLEEVGNQAR;SKMEEQTQQIRLQAEIFQAR;GWFEPIVEDMHR

1000000

Binding and antioxidant activity,

56

IPI00278230

NFU1 iron-sulfur cluster scaffold homolog

131

4.50E-09

5

17

28922

4.92

FIPGKPVLETRTMDFPTPAAAFR;IRPTVQEDGGDVIYRGFEDGIVR

1000000

Organelle and organelle part