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Figure 1 | Parasites & Vectors

Figure 1

From: Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceum

Figure 1

Experimental set up. Sticklebacks were laboratory offspring of a lake and a river population (4 families each, 70–80 individuals per exposure setup). Sticklebacks were sham exposed (0/0), exposed 1 × with 30 (0/1), 5 × pre-exposed with each 20 (5/0) and 5 × pre-exposed with each 20 and 1 × exposed with 30 (5/1) D. pseudospathaceum cercariae. Sticklebacks were investigated for small metacercariae from the last exposure, large metacercariae from the pre-exposures and immune parameters 36 h, 5 days and 15 days after the last exposure with n = 19–32 individuals per treatment group (L = lake, R = river).

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