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Table 2 Main effects of statistical models of the immune parameters of D. pseudospathaceum exposed sticklebacks

From: Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceum

   RLUarea zymosan RLUarea D. p. antigens % S + G2-mlymphocytes % S + G2-mmonocytes
  df χ2 P value χ2 P value χ2 P value χ2 P value
Intercept 1 882.0 < 0.001 750.1 < 0.001 1880.4 < 0.001 2249.9 < 0.001
Family(origin) 6 49.8 < 0.001 45.4 < 0.001 8.1 0.232 9.6 0.145
Sampling time point 1 0.5 0.501 18.6 < 0.001 4.9 0.028 0.2 0.632
Origin 1 20.6 < 0.001 23.7 < 0.001 1.5 0.220 1.1 0.291
Exposure treatment 3 2.9 0.401 0.98 0.804 10.7 0.013 7.2 0.065
Samp. t. x orig. x exp. treat. 10 23.4 0.009 7.2 0.705 7.1 0.716 41.8 < 0.001
  1. Respiratory burst activity (RLUarea) was recorded with zymosan and stimulation with D. pseudospathaceum antigens (Figure 4), and proliferation (% S + G2-m) of lymphocytes and monocytes (Figure 5) was recorded from n = 446 sticklebacks (Figure 1). Data were analysed with Generalized Linear Models (GzLM) with stickleback family nested in stickleback origin (lake/river).