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Table 2 Main effects of statistical models of the immune parameters of D. pseudospathaceum exposed sticklebacks

From: Differences in susceptibility and immune responses of three-spined sticklebacks (Gasterosteus aculeatus) from lake and river ecotypes to sequential infections with the eye fluke Diplostomum pseudospathaceum

  

RLUarea zymosan

RLUarea D. p. antigens

% S + G2-mlymphocytes

% S + G2-mmonocytes

 

df

χ2

P value

χ2

P value

χ2

P value

χ2

P value

Intercept

1

882.0

< 0.001

750.1

< 0.001

1880.4

< 0.001

2249.9

< 0.001

Family(origin)

6

49.8

< 0.001

45.4

< 0.001

8.1

0.232

9.6

0.145

Sampling time point

1

0.5

0.501

18.6

< 0.001

4.9

0.028

0.2

0.632

Origin

1

20.6

< 0.001

23.7

< 0.001

1.5

0.220

1.1

0.291

Exposure treatment

3

2.9

0.401

0.98

0.804

10.7

0.013

7.2

0.065

Samp. t. x orig. x exp. treat.

10

23.4

0.009

7.2

0.705

7.1

0.716

41.8

< 0.001

  1. Respiratory burst activity (RLUarea) was recorded with zymosan and stimulation with D. pseudospathaceum antigens (Figure 4), and proliferation (% S + G2-m) of lymphocytes and monocytes (Figure 5) was recorded from n = 446 sticklebacks (Figure 1). Data were analysed with Generalized Linear Models (GzLM) with stickleback family nested in stickleback origin (lake/river).