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Table 2 Presence of Leishmania confirmed by cytology, parasite culture, Leishmania RT-PCR on single or multiple skin lesions from the dogs studied

From: Papular dermatitis due to Leishmania infantum infection in seventeen dogs: diagnostic features, extent of the infection and treatment outcome

Case number Cytology Culture PCR ( l/s)
1 + NP NP
2 + (a;b;c) + (a;c); − (b); + (a 60000); + (b 20000); + (c 50000);
3 + (a; b) - (a; b) + (a 8000); + (b 20000);
4 + (a; b; c) NP NP
5 - - + (200)
6 - - + (600)
7 - - + (a 5120); + (b 10)
8 + - -
9 - + + (9500)
10 + (a; b) +(a; b) + (a 10000); + (b 6500);
11 NP - + (950)
12 NP NP + (4000)
13 NP NP + (8700)
14 + NP + (1350)
15 + NP + (2000)
16 NP NP + (20)
17 - NP + (800)
  1. +/−: presence or absence of Leishmania amastigotes by cytology or positive or negative by culture or PCR; NP: not performed; (l/s): Leishmania/sample; (a, b or c): results of different lesions sampled.