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Table 3 IFAT, Leishmania RT-PCR and culture results in other tissues at the time of diagnosis and DTH test results at the time of diagnosis and/or during the follow-up

From: Papular dermatitis due to Leishmania infantum infection in seventeen dogs: diagnostic features, extent of the infection and treatment outcome

Case number

IFAT

Blood

Lymph-node

Lymph-node in-vitro culture

Conjunctival swab

Oral swab

DTH

DTH

  

PCR ( Leishmania/mL)

PCR ( Leishmania/sample)

  

PCR ( Leishmania/sample)

48 h (cm)

72 h (cm)

     

PCR ( Leishmania/sample)

   

1

-

-

-

NP

NP

NP

2,50#

1,75#

2

-

-

-

-

NP

NP

NP

NP

3

-

-

-

-

NP

NP

3,00#

3,25#

4

-

-

-

NP

-

NP

1,60#

1,75#

5

1:80

-

-

-

+ (5)

-

2,25*

1,15*

6

-

-

-

-

+ (10)

-

1,25*

2,00*

7

-

-

-

-

-

-

NP

NP

8

-

-

-

-

+ (70)

+ (3)

1,50*

1,25*

9

-

-

-

-

+ (20)

-

1,00*

0,00*

10

-

-

-

-

-

-

1,50*

1,75*

11

1:160

-

-

-

-

-

NP

NP

12

-

-

-

NP

-

-

NP

NP

13

1:80

-

-

NP

NP

NP

NP

NP

14

1:320

-

-

NP

NP

NP

NP

NP

15

1:320

-

-

NP

NP

NP

NP

NP

16

-

-

+ (6)

NP

NP

NP

NP

NP

17

-

-

-

NP

NP

NP

NP

NP

Total number of positive results/total number of dogs studied (%)

5/17 (29.4%)

0/17 (0%)

1/17 (5.8%)

0/9 (0%)

4/9 (44.4%)

1/8 (12.5%)

 
  1. + positive; −: negative; NP: not performed; l/sample: Leishmania/sample; *DTH results upon clinical presentation and in some cases # at follow up.