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Table 3 IFAT, Leishmania RT-PCR and culture results in other tissues at the time of diagnosis and DTH test results at the time of diagnosis and/or during the follow-up

From: Papular dermatitis due to Leishmania infantum infection in seventeen dogs: diagnostic features, extent of the infection and treatment outcome

Case number IFAT Blood Lymph-node Lymph-node in-vitro culture Conjunctival swab Oral swab DTH DTH
   PCR ( Leishmania/mL) PCR ( Leishmania/sample)    PCR ( Leishmania/sample) 48 h (cm) 72 h (cm)
      PCR ( Leishmania/sample)    
1 - - - NP NP NP 2,50# 1,75#
2 - - - - NP NP NP NP
3 - - - - NP NP 3,00# 3,25#
4 - - - NP - NP 1,60# 1,75#
5 1:80 - - - + (5) - 2,25* 1,15*
6 - - - - + (10) - 1,25* 2,00*
7 - - - - - - NP NP
8 - - - - + (70) + (3) 1,50* 1,25*
9 - - - - + (20) - 1,00* 0,00*
10 - - - - - - 1,50* 1,75*
11 1:160 - - - - - NP NP
12 - - - NP - - NP NP
13 1:80 - - NP NP NP NP NP
14 1:320 - - NP NP NP NP NP
15 1:320 - - NP NP NP NP NP
16 - - + (6) NP NP NP NP NP
17 - - - NP NP NP NP NP
Total number of positive results/total number of dogs studied (%) 5/17 (29.4%) 0/17 (0%) 1/17 (5.8%) 0/9 (0%) 4/9 (44.4%) 1/8 (12.5%)  
  1. + positive; −: negative; NP: not performed; l/sample: Leishmania/sample; *DTH results upon clinical presentation and in some cases # at follow up.
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