Papular dermatitis due to Leishmania infantum infection in seventeen dogs: diagnostic features, extent of the infection and treatment outcome

Table 3 IFAT, Leishmania RT-PCR and culture results in other tissues at the time of diagnosis and DTH test results at the time of diagnosis and/or during the follow-up

Case number	IFAT	Blood	Lymph-node	Lymph-node *in-vitro* culture	Conjunctival swab	Oral swab	DTH	DTH
		PCR ( *Leishmania*/mL)	PCR ( *Leishmania*/sample)			PCR ( *Leishmania*/sample)	48 h (cm)	72 h (cm)
					PCR ( *Leishmania*/sample)
1	-	-	-	NP	NP	NP	2,50^#	1,75^#
2	-	-	-	-	NP	NP	NP	NP
3	-	-	-	-	NP	NP	3,00^#	3,25^#
4	-	-	-	NP	-	NP	1,60^#	1,75^#
5	1:80	-	-	-	+ (5)	-	2,25*	1,15*
6	-	-	-	-	+ (10)	-	1,25*	2,00*
7	-	-	-	-	-	-	NP	NP
8	-	-	-	-	+ (70)	+ (3)	1,50*	1,25*
9	-	-	-	-	+ (20)	-	1,00*	0,00*
10	-	-	-	-	-	-	1,50*	1,75*
11	1:160	-	-	-	-	-	NP	NP
12	-	-	-	NP	-	-	NP	NP
13	1:80	-	-	NP	NP	NP	NP	NP
14	1:320	-	-	NP	NP	NP	NP	NP
15	1:320	-	-	NP	NP	NP	NP	NP
16	-	-	+ (6)	NP	NP	NP	NP	NP
17	-	-	-	NP	NP	NP	NP	NP
Total number of positive results/total number of dogs studied (%)	5/17 (29.4%)	0/17 (0%)	1/17 (5.8%)	0/9 (0%)	4/9 (44.4%)	1/8 (12.5%)

+ positive; −: negative; NP: not performed; l/sample: Leishmania/sample; *DTH results upon clinical presentation and in some cases # at follow up.