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Figure 5 | Parasites & Vectors

Figure 5

From: Parasitic antigens alter macrophage polarization during Schistosoma japonicum infection in mice

Figure 5

Differential effects of SWA and SEA on macrophage polarization. Peritoneal macrophages from normal mice were purified and treated as described in Methods. LPS was included as positive controls for classical activation and IL-4 for alternative activation. The expression of CD16/32 and CD206 were evaluated by FCM analysis (A). Representative histograms obtained by FCM analysis (top row). Percentages of CD16/32+ macrophages and CD206+ macrophages (middle row). Mean fluorescence intensity (MFI) of CD16/32 and CD206 expression on macrophages (bottom row). The transcript levels for chemokines (B), cytokines (C) and enzymes of arginine metabolism (D) were evaluated by real-time PCR. The expression levels for each molecule were normalized to the level in macrophages treated with PBS. Data were mean ± SD of three independent experiments.

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