Field comparison of circulating antibody assays versus circulating antigen assays for the detection of schistosomiasis japonica in endemic areas of China

Table 2 Key characteristics of six different diagnostic methods

Assay	Tested samples	Positive samples	Adjusted prevalence (%) [95% CI]	Detection target	Solid phase	Time required per sample	Volume of sample	Extra supplies*
IHA	1864	240	12.9 [11.38-14.42]	Anti-SEA antibody	Red blood cells from sheep	40 min	25 μl	Yes
ELISA	1864	240	12.9 [11.38-14.42]	Anti-SEA antibody	Microtitre plate	90 min	1 μl	Yes
DDIA	1864	231	12.4 [10.90-14.90]	Anti-SEA antibody	Nitrocellulose membrane	15 min	10 μl	No
ELISA (COM)	1864	87	4.7 [3.74-5.66]	Circulating antigen	Microtitre plate	90 min	50 μl	Yes
ELISA (IgY)	1864	81	4.3 [3.40-5.22]	Circulating antigen	Microtitre plate	90 min	20 μl	Yes
Kato-Katz	1864	28	1.5	Egg	Plastic template	40 min	41.7 mg	Yes

*Additional equipment required for all serological tests consisted of handheld micropipettes and micropipette tips.

ISSN: 1756-3305