TgPuf Pumilio homology domain (PUM-HD) organization. (A) A schematic representation of the genomic structure of TgPuf loci (not to scale). Exons are indicated as boxes and introns as solid lines. The conserved RNA-binding domain (RBD) is shown as yellow boxes and the two flanking imperfect pseudo-repeats are shown as blue boxes. (B) The domain organization of predicted TgPuf1 and TgPuf2 proteins. RBDs for each protein are shown with eight repeats (yellow boxes) and two flanking imperfect pseudo-repeats (blue boxes) (not to scale). Puf domain of TgPuf1 is located near the C-terminus (1145–1488 aa), whereas the Puf domain of TgPuf2 is located close to the center of the predicted protein (972–1312 aa).