The measurement of DENV-2 concentration in infected C3H mouse blood (A) dan early-stage infected mosquitoes (B). DENV-2 titer from infected C3H mice (A). DENV-2 titer after virus inoculation to mice intra-peritoneally using our technique. The blood was obtained from the orbital sinus and subjected to plaque assay. The result is the mean (±SE) of three different plaque assay experiments for each time point. DENV-2 titer from early-stage-infected mosquitoes (B). DENV-2 titer after oral virus administration on mosquitoes using our technique. Mosquitoes were randomly picked from a colony of 100–150 females and subjected to plaque assay. The result is the mean (±SE) of three to four individual mosquitoes for each time point.