Construction of recombinant attenuated Salmonella carrying pVAX1-MEG-CTXA
/B plasmid encoding multi-epitope genes derived from T. gondii and A
/B subunits of cholera toxin. (A) The diagram of the construction of recombinant attenuated Salmonella. (B) Gel electrophoresis analysis of pVAX1-MEG-CTXA2/B plasmid encoding multi-epitope genes of Toxoplasma gondii. Lane M1: DNA Marker D, L 100 + 6000, Lane M2: DNA Marker DL2000, Lane1: pVAX1-MEG-CTXA2/B plasmid digested by EcoR I, Lane 2: pVAX1-MEG-CTXA2/B plasmid digested by Hind III and EcoR I, Lane 3: pVAX1-MEG-CTXA2/B digested by Hind III and Kpn I, Lane4: PCR product of CTXA2/B.