Specific humoral response to native T. gondii antigens in mice immunized with multi-epitope DNA vaccine was measured using ELISA 2 weeks after the last immunization. (A) Total Anti-T. gondii IgG; (B) Anti-T. gondii IgA were tested by sera diluted 1:200 from BALB/c mice via three different immunized routes: i.o.( intraoral); i.n. (intranasal); i.m. (intramuscular).