Figure 3

Survival curves of immunized mice after challenge with 10³tachyzoite forms of RH T. gondii strain 4 weeks after the last immunization. (A) Intramuscular immunization group, the mice were injected with 100 μl 1 μg/μl recombinant plasmid, empty plasmid or PBS in the quadriceps muscle; (B) Nasal immunization group, each mouse was given a nasal drip of 50 μl 10⁹ cfu/ml Salmonella bacteria with recombinant plasmid, empty plasmid or empty bacteria; (C) Oral immunization group, the mice were administrated orally with 200 μl 10⁹ cfu/ml Salmonella bacteria with recombinant plasmid, empty plasmid, or empty bacteria.