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Table 2 CD4 + , CD8 + subtypes of T cells from immunized mice were measured using flow cytometry

From: Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice

Immunization routea Immunization regimen CD4+(%)b CD8+(%)b
Intramuscular vaccination PBS 26.71 ± 1.32 21.67 ± 0.82
  pVAX1 25.45 ± 1.13 22.37 ± 0.98
  pVAX1-MEG-CTXA2/B 24.56 ± 1.25 28.54 ± 0.92
Intranasal vaccination BRD509 25.71 ± 0.64 22.37 ± 0.83
  BRD509/pVAX1 24.32 ± 1.75 26.34 ± 0.93
  BRD509/pVAX1-MEG-CTXA2/B 24.68 ± 1.43 30.01 ± 1.78
Intraoral vaccination BRD509 27.45 ± 1.91 24.38 ± 0.98
  BRD509/pVAX1 24.32 ± 1.21 30.28 ± 0.53
  BRD509/pVAX1-MEG-CTXA2/B 25.15 ± 1.55 35.55 ± 0.70
  1. aMice were immunized by three immunization routes, intramuscular, intraoral, intranasal, on day 0 and day 14 and day 28 with different immunization regimens.
  2. bThe splenocyte culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were stained with FITC-labeled anti-mouse CD8+ monoclonal antibody and PE-labeled anti-mouse CD4+ monoclonal antibody, T lymphocyte subsets were measured using flow cytometry.