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Table 3 Cell proliferative assay and cytokine production in the splenocyte cultures obtained from immunized mice

From: Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice

Immunization routea Immunization regimen Cytokine production (pg/ml)b
   IL-2 IFN-γ IL-4 IL-5 SIc
Intramuscular vaccination Saline 12 ± 3 13 ± 8 <10 <10 0.23
  pVAX1 16 ± 4 15 ± 5 11 ± 8 <10 0.51
  pVAX1-MEG-CTXA2/B 93 ± 21 385 ± 64 15 ± 7 13 ± 6 1.61
Intranasal vaccination BRD509 13 ± 4 14 ± 5 <10 17 ± 5 0.75
  BRD509/pVAX1 14 ± 7 15 ± 6 <10 19 ± 5 1.07
  BRD509/ pVAX1-MEG-CTXA2/B 105 ± 30 412 ± 24 23 ± 6 <10 2.12
Intraoral vaccination BRD509 12 ± 5 15 ± 4 <10 16 ± 8 0.75
  BRD509/pVAX1 15 ± 7 18 ± 4 <10 16 ± 7 1.45
  BRD509/ pVAX1-MEG-CTXA2/B 121 ± 36 507 ± 16 <10 12 ± 4 2.85
  1. aMice were immunized by three immunization routes, intramuscular, intraoral, intranasal on day 0 and day 14 and day 28 with different immunization regimens.
  2. bThe splenocyte culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were examined for cytokine production by sandwich ELISA obtained at 24 h for IL-4, at 36 h for IL-5, at 72 h for IL-2 and 96 h for IFN-γ.
  3. cThe results of proliferation assays are expressed as the stimulation index (SI), calculated as the ratio between the mean counts per minute (cpm) for triplicate stimulated cultures and the mean counts per min for triplicate unstimulated cultures. SI values 2.5-fold greater than the SI of the control groups were considered as significant.