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Table 3 Cell proliferative assay and cytokine production in the splenocyte cultures obtained from immunized mice

From: Comparative efficacy of a multi-epitope DNA vaccine via intranasal, peroral, and intramuscular delivery against lethal Toxoplasma gondii infection in mice

Immunization routea

Immunization regimen

Cytokine production (pg/ml)b

  

IL-2

IFN-γ

IL-4

IL-5

SIc

Intramuscular vaccination

Saline

12 ± 3

13 ± 8

<10

<10

0.23

 

pVAX1

16 ± 4

15 ± 5

11 ± 8

<10

0.51

 

pVAX1-MEG-CTXA2/B

93 ± 21

385 ± 64

15 ± 7

13 ± 6

1.61

Intranasal vaccination

BRD509

13 ± 4

14 ± 5

<10

17 ± 5

0.75

 

BRD509/pVAX1

14 ± 7

15 ± 6

<10

19 ± 5

1.07

 

BRD509/ pVAX1-MEG-CTXA2/B

105 ± 30

412 ± 24

23 ± 6

<10

2.12

Intraoral vaccination

BRD509

12 ± 5

15 ± 4

<10

16 ± 8

0.75

 

BRD509/pVAX1

15 ± 7

18 ± 4

<10

16 ± 7

1.45

 

BRD509/ pVAX1-MEG-CTXA2/B

121 ± 36

507 ± 16

<10

12 ± 4

2.85

  1. aMice were immunized by three immunization routes, intramuscular, intraoral, intranasal on day 0 and day 14 and day 28 with different immunization regimens.
  2. bThe splenocyte culture supernatants taken from mice (n = 3, each group) 2 weeks after the last immunization were examined for cytokine production by sandwich ELISA obtained at 24 h for IL-4, at 36 h for IL-5, at 72 h for IL-2 and 96 h for IFN-γ.
  3. cThe results of proliferation assays are expressed as the stimulation index (SI), calculated as the ratio between the mean counts per minute (cpm) for triplicate stimulated cultures and the mean counts per min for triplicate unstimulated cultures. SI values 2.5-fold greater than the SI of the control groups were considered as significant.
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Parasites & Vectors

ISSN: 1756-3305

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