Real-time PCR detection of the HhaI tandem DNA repeat in pre- and post-patent Brugia malayi infections: a study in Indonesian transmigrants

Table 4 Detection of B . malayi HhaI in transmigrants and life-long residents in South Sulawesi, Indonesia

Length of residence	Analyzed individuals	Children/Adults^a	Mf prevalence	*HhaI* PCR prevalence > 53 copies (%)	*HhaI* PCR prevalence < 53 copies (%)^b	IgG4 BmR1 prevalence (%)
≤ 1 month	15	1/14	- ^c	0/15 (0)	12/15 (80)	0/15 (0)
2-4 months	33	10/23	- ^c	8/33 (24)	17/33 (52)	0/33 (0)
3 years	67	34/33	0^d	17/67 (25)	41/67 (61)	5/67 (7)
4 years	52	19/33	0	36/52 (69)	14/52 (27)	6/52 (12)
5 - 6 years	60	28/32	5	36/60 (60)	14/60 (23)	25/60 (42)
LLR	105	71/34	14	65/105 (62)	30/105 (29)	42/105 (40)
Total	334	165/169	19

^aChildren ≤ 15 years, adults 16 years + .
^bAll samples < 2 HhaI copies/μl were considered PCR negative (detection limit), all samples < 53 HhaI copies/μl were considered as not infected.
^cFor logistic reasons blood was collected during daytime in 2 villages, therefore Mf counts are absent.
^dMf prevalence in the 3-year residents could be determined in 20 individuals only.

ISSN: 1756-3305