Figure 2

Alternative splicing of C. elegans gene C05B5.5. (A) Isogroup00600 from our de novo cDNA assembly contains two isotigs derived from C. elegans gene C18E3.6 (exons depicted as blue bars in top track). Alignment of Roche/454 reads (green bars with arrowheads indicating directionality) gave rise to three distinct contigs (dark, medium and light orange bars). These contigs were pieced together to form isotigs 01225 and 01226 based on read support displayed in the contig graph. Isotig01225, which contains all three contigs, corresponds perfectly to the gene model (blue bars). However, isotig01226 includes only the first (light orange) and third (dark orange), which results in a 50 bp gap with respect to isotig01225 and the gene model. (B) Illumina RNAseq reads (dark purple, horizontal bars) mapped to isotig01226 further verifies the junction between the first (light orange) and third (dark orange) contigs, with proportional coverage indicated (light purple, vertical bars). This figure was adapted from alignments visualized using the Integrated Genomics Viewer [48, 49].