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Table 1 Ae. aegypti females positive for dengue infection; ranges of optical density and cycle threshold plus RNA copies using Platelia Dengue NS1 Ag-ELISA and qRT-PCR, respectively, in detecting DENV-2 between 0–72 hours after mosquitoes were killed and maintained at insectary temperatures

From: Preliminary evaluation on the efficiency of the kit Platelia Dengue NS1 Ag-ELISA to detect dengue virus in dried Aedes aegypti: a potential tool to improve dengue surveillance

Time points (h)

DENV-2 detection (NS1)

DENV-2 detection (qRT-PCR)

Positives (%)

Optical density range

Positives (%)

Cycle threshold range

RNA copy range

0

6 (60)

0.101 – 2.874

6 (60)

38.05 – 42.98

2278.06 – 14112.42

6

7 (70)

0.116 – 3.58

7 (70)

36.78 – 43.87

1641.77 – 22576.03

12

8 (80)

0.101 – 3.003

4 (40)

37.99 – 44.36

1367.33 – 14413.18

24

7 (70)

0.111 – 3.92

4 (40)

39.12 – 43.64

1946.2 – 9519.86

48

5 (50)

0.099 – 2.876

1 (10)

42.83

2410.76

72

5 (50)

0.156 – 3.071

1 (10)

40.36

6525.18

Total

38 (63.3)

23 (38.3)