Implications of low-density microfilariae carriers in Anopheles transmission areas: molecular forms of Anopheles gambiae and Anopheles funestus populations in perspective

Table 1 Oligonucleotide primer sequences and PCR reaction conditions for species identification

Primer for species ID	Sequence 5’ ⇒ 3’	PCR product size (bp)	PCR conditions
*Anopheles gambiae* s.l. species
Universal primer	GTGTGCCCCTTCCTCGATGT	468	93°C 3’ followed by 35 cycles (93°C 30”; 50°C 30”; 72°C 1’); 93°C 30”; 50°C 30”; 72°C 10’
Anopheles gambiae s.s.	CTGGTTTGGTCGGCACGTTT	390
Anopheles merus/melax	TGACCAACCCACTCCCTTGA	464
Anopheles arabiensis	AAGTGTCCTTCTCCATCCTA	315
Anopheles quadrianulatus	CAGACCAAGATGGTTAGTAT	153
*Anopheles funestus* s.l. species
Universal primer	TGTGAACTGCAGGACACAT		30 cycles (94°C 30”; 40°C 30”; 72°C 30”); 72°C 10’
Anopheles funestus s.s.	GCATCGATGGGTTAATCATG	460
Anopheles vaneedeni	TGTCGACTTGGTAGCCGAAC	555
Anopheles rivulorum	CAAGCCGTTCGACCCTGATT	400
Anopheles parensis	TGCGGTCCCAAGCTAGGTTC	235
Anopheles leesoni	TACACGGGCGCCATGTAGTT	146
*Wuchereria bancrofti*
NV-1	CGTGATGGCATCAAAGTAGCG	188	94°C 3’; followed by 35 cycles (94°C 1’; 55°C 1’; 72°C 2’); 94°C 1’; 55°C 1’; 72°C 10”
NV-2	CCCTCACTTACCATAAGACAAC	188

ISSN: 1756-3305