Figure 1

Diagram of the experimental design used in this study. Treatments are abbreviated as follows: multiple-bloodfed non-oviposition-deprived control group (MBC; red), multiple-bloodfed oviposition-deprived (MBOD; blue), once-bloodfed oviposition-deprived (OBOD; green), and blood-deprived age control (BD; black). 150 newly-emerged females were placed in each cage at the start of the experiment. The boxed area and dotted arrows indicate that these steps were repeated weekly until the last mosquito in the experiment died.