A | Mortality (L3 larvae) |
---|
| X ± SD | % |
---|
Control | a | 0 |
Control 2 | b | 0 |
12 h | 1.66 ± 1.15 ab** | 17 |
24 h | 1.0 ± 0 | 10 |
48 h | 1.0 ± 0 | 3 |
72 h | 0 | 0 |
96 h | 0 | 0 |
120 h | 0 | 0 |
B
|
Mortality (L3 larvae)
|
|
Number
|
%
|
Control | 0 | 0 |
Control 2 | 0 | 0 |
1 h | 0 | 0 |
2 h | 0 | 0 |
3 h | 0 | 0 |
24 h | 0 | 0 |
48 h | 60 | 100 |
- After 12-120 h (4A) and 1-48 h (4B) incubation, larvae in mid-development were treated with burchellin. Treatment of A. aegypti with 15 μg/mL burchellin using 10 L3 larvae per group (4A) and 30 μg/mL burchellin using 100 larvae (L3) per group (4B). Larvae (n = 10) were removed at intervals of 1 - 48 h (4B). All experiments were performed in triplicate. Values are mean ± standard deviation (X ± SD). Values followed by the same letter did not differ significantly; Tukey test, P > 0.05. Significance levels are represented as **P < 0.01 vs. Control 2 = Acetone control.