After 12-120 h (4A) and 1-48 h (4B) incubation, larvae in mid-development were treated with burchellin. Treatment of A. aegypti with 15 μg/mL burchellin using 10 L3 larvae per group (4A) and 30 μg/mL burchellin using 100 larvae (L3) per group (4B). Larvae (n = 10) were removed at intervals of 1 - 48 h (4B). All experiments were performed in triplicate. Values are mean ± standard deviation (X ± SD). Values followed by the same letter did not differ significantly; Tukey test, P > 0.05. Significance levels are represented as **P < 0.01 vs. Control 2 = Acetone control.