Figure 2

Multiple alignment of deduced amino acid sequences of CS from T. rangeli and other representative organisms. The identity (black background) and conservation (grey background) of the amino acid residues are shown. Brackets indicate the consensus amino acid residues of the putative pyridoxal phosphate-binding motif (PXXSVKDR); the substitute for the proline residue is marked with (•), and the lysine residues required for cysteine synthase activity are indicated with triangles. The β8–β9 loop at the entrance of the active site is indicated with an asterisk (*), and the positively charged residues involved in binding with SAT are indicated with (+). TraCS: Trypanosoma rangeli; TcCS: Trypanosoma cruzi (Tc00.1047053507165.50); LbrCS: Leishmania braziliensis (LbrM.35.3820); LmjCS: Leishmania major (LmjF.36.3590); EhCS: Entamoeba histolytica; TvCS: Trichomonas vaginalis (XP001325874); StCS A: Salmonella typhimurium CysK (P0A1E4); StCS B: Salmonella typhimurium CysM (NP_456975).