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Table 3 DNA extraction and molecular marker identification methods used by laboratories in each of the ring trials

From: Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia

Code

RT1

RT2a

RT2b

RT3

 

Extraction1

Marker used2

Identification

Marker used

Extraction3

Marker used

LabA

Qiagen Kit

ITS1

ITS1

ND

ND

LabB

Crude lysate

ITS1

ITS1

ND

ND

LabC

Qiagen Kit

ITS1

ITS1

ITS1

Qiagen Kit

COI and ITS1

LabD

MN Kit

ITS1

ITS1

ITS1

MN Kit

ITS1

LabE

Crude lysate

COI

COI

COI

Qiagen Kit

COI

LabF

Roche Kit

COI

COI

ITS1

Roche Kit

ITS1

LabG

Chelex

COI

COI

COI

Chelex

COI

LabH

ND

ND

ITS1

Squish Kit

ITS1

LabI

ND

ND

ITS1

Qiagen Kit

ITS1

LabJ

Crude lysate

ITS1 (MA)

ITS1 (MA)

ITS1 (MA)

Chelex

ITS1 (MA)

LabK

Crude lysate

ITS2/ITS1

ITS2/ITS1

ITS2/ITS1

IQCasework

ITS2/ITS1

LabL

AJ Kit

COI

COI

COI

Qiagen Kit

COI

  1. 1Qiagen Kit: DNeasy Tissue and Blood kit, Qiagen, USA; MN Kit: NucleoSpin® Tissue, Macherey-Nagel; Roche Kit: High Pure PCR Template Preparation Kit, Roche; Chelex: Chelex 100 chelating ion exchange resin, Bio-Rad; AJ Kit: innuPREP DNA Mini Kit, Analytik Jena; NA: not applicable; ND: not done.
  2. 2MA: microarray.
  3. 3Squish Kit: Squish buffer; IQCasework: DNA IQ Casework Sample Kit, Promega.