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Table 4 Sensitivity, lure specificity and Obsoletus specificity for assays using COI and ITS1 markers

From: Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia

Marker Tested effect Lab n Sensitivity n Lure specificity n Obsoletus specificity
COI Overall 5 240 59.6 52 92.3 441 99.1
  Laboratory 5 240 65.6 [48.4-81.2] 52 92.3 [85.7-100.0] 441 99.1 [97.6-100.0]
  Species C. obsoletus 5 60 61.7 - - 107 99.1
   C. scoticus 5 60 41.7 - - 121 99.2
   C. dewulfi 5 60 70.0 - - 103 100.0
   C. chiopterus 5 60 65.0 - - 108 98.1
  Sample type Ground midge 4 112 33.0 24 95.8 114 99.1
   Whole midge 4 64 79.7 16 100.0 156 99.4
   DNA sample 4 64 85.9 12 75.0 171 98.8
  DNA extraction method Crude lysate 1 28 53.6 6 100.0 45 100.0
   Chelex method 1 44 34.1 10 100.0 48 97.9
   Commercial kits 3 168 67.9 [3.6-83.0] 36 87.5 [83.3-100.0] 345 100.0 [99.0-100.0]
ITS1 Overall 7 288 76.4 64 81.2 795 94.7
  Laboratory 7 288 79.7 [40.6-100.0] 64 100.0 [42.9-100.0] 795 95.0 [83.3-100.0]
  Species C. obsoletus 7 72 81.9 - - 198 96.5
   C. scoticus 7 72 80.6 - - 203 86.2
   C. dewulfi 7 72 79.2 - - 197 97.5
   C. chiopterus 7 72 63.9 - - 197 98.9
  Sample type Ground midge 4 112 64.3 24 70.8 309 90.6
   Whole midge 5 80 81.2 20 75.0 198 100.0
   DNA sample 7 96 86.5 20 100.0 288 95.5
  DNA extraction method Crude lysate 1 28 39.3 6 100.0 78 83.3
   Squish buffer 1 16 56.2 4 100.0 27 100.0
   Commercial kits 5 244 81.5 [77.3-100.0] 54 90.0 [20.0-100.0] 661 95.6 [95.0-100.0]
ITS2/ITS1 Overall 1 64 90.6 14 100.0 177 99.4
  Species C. obsoletus / 33 97.0    90 100.0
   C. scoticus / 32 87.5    94 98.9
   C. dewulfi / 31 100.0    92 100.0
   C. chiopterus / 32 96.9    92 100.0
  Sample type Ground midge / 56 92.9 6 100.0 156 100.0
   Whole midge / 32 93.8 4 100.0 93 98.9
   DNA sample / 40 100.0 4 100.0 120 100.0
Microarray Overall 1 64 100.0 14 100.0 192 100.0
  1. Probabilities (expressed as percentages) are written in bold font when the corresponding effect was significantly different from 0 (α = 0.05). Minimum and maximum values are shown in brackets. DNA extraction method is not tested for both ITS2/ITS1 and microarray because one method was used. For the microarray, the number of samples tested is the same as for the ITS2/ITS1.