Marker | Tested effect | Lab | n | Sensitivity | n | Lure specificity | n | Obsoletus specificity | |
---|---|---|---|---|---|---|---|---|---|
COI | Overall | 5 | 240 | 59.6 | 52 | 92.3 | 441 | 99.1 | |
Laboratory | 5 | 240 | 65.6 [48.4-81.2] | 52 | 92.3 [85.7-100.0] | 441 | 99.1 [97.6-100.0] | ||
Species | C. obsoletus | 5 | 60 | 61.7 | - | - | 107 | 99.1 | |
C. scoticus | 5 | 60 | 41.7 | - | - | 121 | 99.2 | ||
C. dewulfi | 5 | 60 | 70.0 | - | - | 103 | 100.0 | ||
C. chiopterus | 5 | 60 | 65.0 | - | - | 108 | 98.1 | ||
Sample type | Ground midge | 4 | 112 | 33.0 | 24 | 95.8 | 114 | 99.1 | |
Whole midge | 4 | 64 | 79.7 | 16 | 100.0 | 156 | 99.4 | ||
DNA sample | 4 | 64 | 85.9 | 12 | 75.0 | 171 | 98.8 | ||
DNA extraction method | Crude lysate | 1 | 28 | 53.6 | 6 | 100.0 | 45 | 100.0 | |
Chelex method | 1 | 44 | 34.1 | 10 | 100.0 | 48 | 97.9 | ||
Commercial kits | 3 | 168 | 67.9 [3.6-83.0] | 36 | 87.5 [83.3-100.0] | 345 | 100.0 [99.0-100.0] | ||
ITS1 | Overall | 7 | 288 | 76.4 | 64 | 81.2 | 795 | 94.7 | |
Laboratory | 7 | 288 | 79.7 [40.6-100.0] | 64 | 100.0 [42.9-100.0] | 795 | 95.0 [83.3-100.0] | ||
Species | C. obsoletus | 7 | 72 | 81.9 | - | - | 198 | 96.5 | |
C. scoticus | 7 | 72 | 80.6 | - | - | 203 | 86.2 | ||
C. dewulfi | 7 | 72 | 79.2 | - | - | 197 | 97.5 | ||
C. chiopterus | 7 | 72 | 63.9 | - | - | 197 | 98.9 | ||
Sample type | Ground midge | 4 | 112 | 64.3 | 24 | 70.8 | 309 | 90.6 | |
Whole midge | 5 | 80 | 81.2 | 20 | 75.0 | 198 | 100.0 | ||
DNA sample | 7 | 96 | 86.5 | 20 | 100.0 | 288 | 95.5 | ||
DNA extraction method | Crude lysate | 1 | 28 | 39.3 | 6 | 100.0 | 78 | 83.3 | |
Squish buffer | 1 | 16 | 56.2 | 4 | 100.0 | 27 | 100.0 | ||
Commercial kits | 5 | 244 | 81.5 [77.3-100.0] | 54 | 90.0 [20.0-100.0] | 661 | 95.6 [95.0-100.0] | ||
ITS2/ITS1 | Overall | 1 | 64 | 90.6 | 14 | 100.0 | 177 | 99.4 | |
Species | C. obsoletus | / | 33 | 97.0 | 90 | 100.0 | |||
C. scoticus | / | 32 | 87.5 | 94 | 98.9 | ||||
C. dewulfi | / | 31 | 100.0 | 92 | 100.0 | ||||
C. chiopterus | / | 32 | 96.9 | 92 | 100.0 | ||||
Sample type | Ground midge | / | 56 | 92.9 | 6 | 100.0 | 156 | 100.0 | |
Whole midge | / | 32 | 93.8 | 4 | 100.0 | 93 | 98.9 | ||
DNA sample | / | 40 | 100.0 | 4 | 100.0 | 120 | 100.0 | ||
Microarray | Overall | 1 | 64 | 100.0 | 14 | 100.0 | 192 | 100.0 |