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Table 4 Sensitivity, lure specificity and Obsoletus specificity for assays using COI and ITS1 markers

From: Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting four species of the subgenus Avaritia

Marker

Tested effect

Lab

n

Sensitivity

n

Lure specificity

n

Obsoletus specificity

COI

Overall

5

240

59.6

52

92.3

441

99.1

 

Laboratory

5

240

65.6 [48.4-81.2]

52

92.3 [85.7-100.0]

441

99.1 [97.6-100.0]

 

Species

C. obsoletus

5

60

61.7

-

-

107

99.1

  

C. scoticus

5

60

41.7

-

-

121

99.2

  

C. dewulfi

5

60

70.0

-

-

103

100.0

  

C. chiopterus

5

60

65.0

-

-

108

98.1

 

Sample type

Ground midge

4

112

33.0

24

95.8

114

99.1

  

Whole midge

4

64

79.7

16

100.0

156

99.4

  

DNA sample

4

64

85.9

12

75.0

171

98.8

 

DNA extraction method

Crude lysate

1

28

53.6

6

100.0

45

100.0

  

Chelex method

1

44

34.1

10

100.0

48

97.9

  

Commercial kits

3

168

67.9 [3.6-83.0]

36

87.5 [83.3-100.0]

345

100.0 [99.0-100.0]

ITS1

Overall

7

288

76.4

64

81.2

795

94.7

 

Laboratory

7

288

79.7 [40.6-100.0]

64

100.0 [42.9-100.0]

795

95.0 [83.3-100.0]

 

Species

C. obsoletus

7

72

81.9

-

-

198

96.5

  

C. scoticus

7

72

80.6

-

-

203

86.2

  

C. dewulfi

7

72

79.2

-

-

197

97.5

  

C. chiopterus

7

72

63.9

-

-

197

98.9

 

Sample type

Ground midge

4

112

64.3

24

70.8

309

90.6

  

Whole midge

5

80

81.2

20

75.0

198

100.0

  

DNA sample

7

96

86.5

20

100.0

288

95.5

 

DNA extraction method

Crude lysate

1

28

39.3

6

100.0

78

83.3

  

Squish buffer

1

16

56.2

4

100.0

27

100.0

  

Commercial kits

5

244

81.5 [77.3-100.0]

54

90.0 [20.0-100.0]

661

95.6 [95.0-100.0]

ITS2/ITS1

Overall

1

64

90.6

14

100.0

177

99.4

 

Species

C. obsoletus

/

33

97.0

  

90

100.0

  

C. scoticus

/

32

87.5

  

94

98.9

  

C. dewulfi

/

31

100.0

  

92

100.0

  

C. chiopterus

/

32

96.9

  

92

100.0

 

Sample type

Ground midge

/

56

92.9

6

100.0

156

100.0

  

Whole midge

/

32

93.8

4

100.0

93

98.9

  

DNA sample

/

40

100.0

4

100.0

120

100.0

Microarray

Overall

1

64

100.0

14

100.0

192

100.0

  1. Probabilities (expressed as percentages) are written in bold font when the corresponding effect was significantly different from 0 (α = 0.05). Minimum and maximum values are shown in brackets. DNA extraction method is not tested for both ITS2/ITS1 and microarray because one method was used. For the microarray, the number of samples tested is the same as for the ITS2/ITS1.