Table 1 Sequences of the oligonucleotide primers used in this study
From: Molecular identification of Theileria parasites of northwestern Chinese Cervidae
Pathogen | Target gene | Oligonucleotide sequences (5′-3′) | Final amplicon size (bp) | Reference | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
Hemoparasite | 18S rRNA | AACCTGGTTGATCCTGCCAGT | GATCCTTCTGCAGGTTCACCTAC | 1750 | Medlin et al., [20] |
Theileria spp. | 18S rRNA | AGTTTCTGACCTATCAG | TTGCCTTAAACTTCCTTG | 1098 | Allosop et al., [21] |
T. annulata | 30 kDa | GTAACCTTTAAAAACGT | GTTACGAACATGGGTTT | 721 | d’Oliveria et al., [22] |
T. sergenti | MPSP | CACGCTATGTTGTCCAAGAG | TGTGAGACTCAATGCGCCTA | 875 | Liu et al., [23] |
T. sinensis | MPSP | CACTGCTATGTTGTCCAAGAGATATT | AATGCGCCTAAAGATAGTAGAAAAC | 887 | Liu et al., [23] |
T. orientalis | MPSP | CTTTGCCTAGGATACTTCCT | ACGGCAAGTGGTGAGAACT | 776 | Ota et al., [24] |
T. cervi (Type F) | 18S rRNA | TTCCCTTTGAGGGGT | AAGCCTATTCCCGTACC | 654 | Boes et al., [25] |
T. cervi (Type G) | 18S rRNA | CTTCCCGTTATGGAGG | CTTAACCTATTCCCGTGAG | 654 | Boes et al., [25] |
T. luwenshuni | 18S rRNA | GGTAGGGTATTGGCCTACTGA | TCATCCGGATAATACAAGT | 340 | Yin et al., [26] |
T. uilenbergi | 18S rRNA | GGTAGGGTATTGGCCTACCGG | ACACTCGGA AAATGCAAGCA | 340 | Yin et al., [26] |
T. ovis | 18S rRNA | TCGA-GACCTTCGGGT | TCCGGACATTGTAAAACAAA | 520 | Altay et al., [27] |
T. lestoquardi | 18S rRNA | GTGCCGCAAGTGAGTCA | GGACTGATGAGAAGACGATGAG | 785 | Aktas et al., [28] |
T. capreoli | 18S rRNA | gtcttgtaattggaatgatgg | ccaaagactttgatttctctc | 580 | Slemenda et al., [29] |
Babesia spp. | 18S rRNA | TGTCTTGAATACTT(C/G)AGCATGGAA | CGACTTCTCCTTTAAGTGATAAC | 950 | Ramos et al., [10] |
B. odocoilei | 18S rRNA | CACCGTATTTTGACTTTTTGTCGACTGTCGG | CCCGTAACGGACGAACCTTCTCACGGG | 900 | Ramos et al., [10] |
B. bovis | 18S rRNA | CTGTCGTACCGTTGGTTGAC | CGCACGGACGGAGACCGA | 541 | Guido et al., [30] |
B. ovata | AMA-1 | GATACGAGGCTGTCGGTAGC | AGTATAGGTGAGCATCAGTG | 504 | Sivakumar et al., [31] |
B. bigemina | 18S rRNA | TGGCGGCGTTTATTAGTTCG | CCACGCTTGAAGCACAGGA | 1124 | Guido et al., [30] |
B. ovis | 18S rRNA | TGGGCAGGACCTTGGTTCTTCT | CCGCGTAGCGCCGGCTAAATA | 549 | Aktas et al., [32] |
B. motasi | 18S rRNA | AAGAATTTCACCTCTGACAG | GCTTGCTTTTTGTTACTTTG | 179 | Shayan et al., [33] |