Specificity and reliability of the LAMP assay in different insects. To determine the specificity and reliability of our assay we amplified the DNA from different mosquito species (Diptera), including those naturally infected with Wolbachia: (A) Ae. albopictus (1), Ae. fluviatilis (2), Culex sp. (6) those with transinfections; Ae. aegypti – w Mel (3), Ae. aegypti – w MelPop (4), and those with no infection; Ae. aegypti (5), An. aquasalis (7). We also examined insects from a range of different orders that were known to be infected with Wolbachia: (B) Hymenoptera (8), Hemiptera (9), Lepidoptera (10), Orthoptera (11), Siphonaptera (12), Coleoptera (13), Isoptera (14). M = 1 Kb Plus, Invitrogen. NC = negative control. All Wolbachia-infected specimens amplified, while no amplification was observed for uninfected specimens indicating that the assay was both highly reliable and highly specific.