Figure 3From: Wolbachia detection in insects through LAMP: loop mediated isothermal amplificationDetection of Wolbachia infection by ion indicator visualization. (A) With the addition of the ion indicator Hydroxy Naphtol Blue to the LAMP reaction it was possible to determine whether a mosquito specimen was Wolbachia-infected (blue), or -uninfected (purple) using the naked eye. The assay was highly specific with Wolbachia- infected Ae. fluviatilis (1), Culex spp. (2), Ae. aegypti (4), all giving a positive result, while the uninfected An. aquasalis (3) did not. (B) Ethidium bromide stained gel with (A) samples NC = negative control.Back to article page