Antibacterial activity of the anterior midgut contents of Rhodnius prolixus on different days after feeding. A- Activity of anterior midgut contents against E. coli. B- Activity of anterior midgut contents against S. aureus. Antibacterial activity detected by turbidometric assay (TB) (OD550 nm) after 19 h incubation of anterior midgut content samples with different bacteria. Values represent the means ± SD of 9 pools using 3 insects each (n = 27) in triplicate wells. Asterisks relate to significant differences (*p < 0.05, **p < 0.01, ***p < 0.001) obtained after data were compared to day seven using one way ANOVA and Mann Whitney tests.