Humoral responses in Rhodnius prolixus: bacterial feeding induces differential patterns of antibacterial activity and enhances mRNA levels of antimicrobial peptides in the midgut

Table 1 List of primers used in the present study

Gene/name	Sequence 5’-3’	Tm (°C)	Amplicon length
RPDEFAF	GAATACTCCACTCAACCGCAAC	62.7
RPDEFAR	TAGTTCCTTTACATCGGCCA	58.4	295 bp
RPDEFBF	CAGTACCTAGGATATTCCACTCAAC	62.9
RPDEFBR	TAGTTCCTTTACAATGGCCG	58.4	304 bp
RPDEFCF	CAGTACAGTCCTAATACCTAGCC	62.8
RPDEFCR	CAGTTCCTACGCAACGGCCT	64.5	300 bp
RPLYS1F	TTCTTACTGGCTATTTTCGCC	58.7
RPLYS1R	CGACCTCTGCAATGGTACTG	62.4	377 bp
RPLYS2F	CTAGTTTTAACACTATTGCTGCTG	59.4
RPLYS2R	GCCCTTACATTTCTTGATCC	58.4	378 bp
RPPROLF	CTATAACGAGTGAACTATAAGACAA	50.0
RPPROLR	GTGTTTAATGGCGGTAACAAATTAC	53.2	406 bp
RPACTF	CACGAGGCTGTATACAATTCCA	60.8
RPACTR	GTAGCTGTTTAGAAGCATTTGCG	61.0	314 bp

ISSN: 1756-3305