Table 1 Target genes, primers sequence, PCR methods used for pathogens identification
Pathogens | Method | Target gene | Primers sequence (5′-3′) | Product size (bp) | Reference |
|---|---|---|---|---|---|
Babesia/Theileria | Nested PCR | 18S rRNA | RIB-19(CGGGATCCAACCTGGTTGATCCTGC) | 1700 | [28] |
RIB-20(CCGAATTCCTTGTTACGACTTCTC) | |||||
BAB-F(ACCTCACCAGGTCCAGACAG) | 430 | ||||
BAB-R(GTACAAAGGGCAGGGACGTA) | |||||
Anaplasma/Ehrlichia | Nested PCR | 16S rRNA | Eh-out1 (TTGAGAGTTTGATCCTGGCTCAGAACG) | 653 | [29] |
Eh-out2 (CACCTCTACACTAGGAATTCCGCTATC) | |||||
Eh-gs1 (GTAATACTGTATAATCCCTG) | 282 | Â | |||
Eh-gs2 (TATAGGTACCGTCATTATCTTCCCTAC) | |||||
TBEV | Nested PCR | Non-structural protein NS5 | FSM1 (GAGGCTGAACAACTGCACGA) | 357 | [32] |
FSM2 (GAACACGTCCATTCCTGATCT) | |||||
FSM1i (ACGGAACGTGACAAGGCTAG) | 251 | ||||
FSM2i (GCTTGTTACCATCTTTGGAG) | |||||
Rickettsia spp. | Nested PCR | groEL | Gro1 (AAGAAGGCGTGATAAC) | 200 | [30] |
Gro2 (ACTTCCGTAGCACC) | |||||
SF (GATAGAAGAAAAGCAATGATG) | 250 | ||||
SR (CAGCTATTTGAGATTTAATTTG) | |||||
B. burgdorferi s. l. | Nested PCR | Flagellin | Outer1 (CTGCTGGCATGGGAGTTTCT) | 730 | [31] |
Outer2 (TCAATTGCATACTCAGTACT) | |||||
Inner1 (AAGGAATTGGCAGTTCAATC ) | 290 | ||||
Inner2 (ACAGCAATAGCTTCATCTTG ) | |||||
Leishmania infantum | PCR | kDNA | RV1(CTTTTCTGGTCCCGCGGGTAGG) | 183 | [27] |
| Â | Â | Â | RV2(CCACCTGGCCTATTTTACACCA) | Â | Â |