In situ hybridization to appendages of animals of different ages. Most common is greater hybridization for CPF3 than CPLCG3 in P24 (A, B). But occasionally there is similar hybridization of both at P24 (C, D). After eclosion, CPLCG3 is generally stronger in appendages than CPF3, here the antenna of a young adult male, 17–19 h post-eclosion (E, F). ms, muscle; ant, antenna.